ArticleDevelopmental biology2016
Protein tyrosine kinase 7 is essential for tubular morphogenesis of the Wolffian duct.
Article in Developmental biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- We, the developing rete testis, efferent ducts, and Wolffian duct, all hereby agree that we need to connect.Andrology · 2019Pooled it
- The Rho GEF Trio functions in contact inhibition of locomotion of neural crest cells by interacting with Ptk7.Development (Cambridge, England) · 2025Article
- Biomechanical properties of the capsule and extracellular matrix play a major role during the Wolffian/epididymal duct development.Andrology · 2025Article
- Molecular Pathways Implicated in the Differentiation and Function of Epididymal Basal Cells.Advances in experimental medicine and biology · 2025Review
- Local and global changes in cell density induce reorganisation of 3D packing in a proliferating epithelium.Development (Cambridge, England) · 2024Article
- Collective cell dynamics and luminal fluid flow in the epididymis: A mechanobiological perspective.Andrology · 2024Review
- Epithelial and mesenchymal fate decisions in Wolffian duct development.Trends in endocrinology and metabolism: TEM · 2023Review
- Hedgehog signaling regulates Wolffian duct development through the primary cilium†.Biology of reproduction · 2023Article
- Article
- Crucial Convolution: Genetic and Molecular Mechanisms of Coiling during Epididymis Formation and Development in Embryogenesis.Journal of developmental biology · 2022Review
- Planar cell polarity pathway in kidney development, function and disease.Nature reviews. Nephrology · 2021Review
- Mechanical Feedback Control for Multicellular Tissue Size Maintenance: A Minireview.Frontiers in cell and developmental biology · 2021Review
- Convergent extension in mammalian morphogenesis.Seminars in cell & developmental biology · 2020Review
- Polarized cellular mechano-response system for maintaining radial size in developing epithelial tubes.Development (Cambridge, England) · 2019Article
- Exome sequencing of fetal anomaly syndromes: novel phenotype-genotype discoveries.European journal of human genetics : EJHG · 2019Article
- Wnt11 directs nephron progenitor polarity and motile behavior ultimately determining nephron endowment.eLife · 2018Article
- Protein tyrosine kinase 7 regulates extracellular matrix integrity and mesenchymal intracellular RAC1 and myosin II activities during Wolffian duct morphogenesis.Developmental biology · 2018Article
- Cell migration, intercalation and growth regulate mammalian cochlear extension.Development (Cambridge, England) · 2017Article
- PTK7 Faces the Wnt in Development and Disease.Frontiers in cell and developmental biology · 2017Review
- Is the Epididymis a Series of Organs Placed Side By Side?Biology of reproduction · 2016Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The Wolffian duct, the proximal end of the mesonephric duct, undergoes non-branching morphogenesis to achieve an optimal length and size for sperm maturation. It is important to examine the mechanisms by which the developing mouse Wolffian duct elongates and coils for without proper morphogenesis, male infertility will result. Here we show that highly proliferative epithelial cells divide in a random orientation relative to the elongation axis in the developing Wolffian duct. Convergent extension (CE)-like of cell rearrangements is required for elongating the duct while maintaining a relatively unchanged duct diameter. The Wolffian duct epithelium is planar polarized, which is characterized by oriented cell elongation, oriented cell rearrangements, and polarized activity of regulatory light chain of myosin II. Conditional deletion of protein tyrosine kinase 7 (PTK7), a regulator of planar cell polarity (PCP), from mesoderm results in loss of the PCP characteristics in the Wolffian duct epithelium. Although loss of Ptk7 does not alter cell proliferation or division orientation, it affects CE and leads to the duct with significantly shortened length, increased diameter, and reduced coiling, which eventually results in loss of sperm motility, a key component of sperm maturation. In vitro experiments utilizing inhibitors of myosin II results in reduced elongation and coiling, similar to the phenotype of Ptk7 knockout. This data suggest that PTK7 signaling through myosin II regulates PCP, which in turn ensures CE-like of cell rearrangements to drive elongation and coiling of the Wolffian duct. Therefore, PTK7 is essential for Wolffian duct morphogenesis and male fertility.
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Registered trials
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