ArticlePLoS genetics2016
Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling.
Article in PLoS genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 86 citations in OpenAlex.
- Dry side of the core: a meta-analysis addressing the original nature of the ABA signalosome at the onset of seed imbibition.Frontiers in plant science · 2023Pooled it
- Protein Phosphorylation: An Essential Role in Shoot Apical Meristem Homeostasis.Plants (Basel, Switzerland) · 2026Review
- GWAS reveal SUBER GENE1-mediated suberization via type one phosphatases.Nature plants · 2026Article
- Type one protein phosphatases (TOPPs) catalyze EIN2 dephosphorylation to regulate ethylene signaling inScience advances · 2026Article
- Protein Post-Translational Modifications in Plant Abiotic Stress Responses.Plants (Basel, Switzerland) · 2025Review
- GhTOPP4aD and GhRAF36 inversely regulate cotton (Gossypium hirsutum) response to ABA and salt stress through reversible phosphorylation of GhABI1.Plant biotechnology journal · 2025Article
- Arabidopsis CNL receptor SUT1 confers immunity in hydathodes against the vascular pathogen Xanthomonas campestris pv. campestris.PLoS pathogens · 2025Article
- The Wheat Intrinsically Disordered ProteinBiomolecules · 2025Article
- Type One Protein Phosphatase 4aD Negatively Regulates Cotton (International journal of molecular sciences · 2025Article
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- The rice SnRK family: biological roles and cell signaling modules.Frontiers in plant science · 2023Review
- Genetic mapping identifies genomic regions and candidate genes for seed weight and shelling percentage in groundnut.Frontiers in genetics · 2023Article
- Molecular Mechanisms UnderlyingInternational journal of environmental research and public health · 2022Article
- Type one protein phosphatase regulates fixed-carbon starvation-induced autophagy in Arabidopsis.The Plant cell · 2022Article
- Beyond stress response: OST1 opening doors for plants to grow.Stress biology · 2022Review
- NUA and ESD4 negatively regulate ABA signaling during seed germination.Stress biology · 2022Article
- The tomato OST1-VOZ1 module regulates drought-mediated flowering.The Plant cell · 2022Article
- Rapeseed (Frontiers in plant science · 2022Article
- Height to first pod: A review of genetic and breeding approaches to improve combine harvesting in legume crops.Frontiers in plant science · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
Abstract
The phytohormone abscisic acid (ABA) regulates plant growth, development and responses to biotic and abiotic stresses. The core ABA signaling pathway consists of three major components: ABA receptor (PYR1/PYLs), type 2C Protein Phosphatase (PP2C) and SNF1-related protein kinase 2 (SnRK2). Nevertheless, the complexity of ABA signaling remains to be explored. To uncover new components of ABA signal transduction pathways, we performed a yeast two-hybrid screen for SnRK2-interacting proteins. We found that Type One Protein Phosphatase 1 (TOPP1) and its regulatory protein, At Inhibitor-2 (AtI-2), physically interact with SnRK2s and also with PYLs. TOPP1 inhibited the kinase activity of SnRK2.6, and this inhibition could be enhanced by AtI-2. Transactivation assays showed that TOPP1 and AtI-2 negatively regulated the SnRK2.2/3/6-mediated activation of the ABA responsive reporter gene RD29B, supporting a negative role of TOPP1 and AtI-2 in ABA signaling. Consistent with these findings, topp1 and ati-2 mutant plants displayed hypersensitivities to ABA and salt treatments, and transcriptome analysis of TOPP1 and AtI-2 knockout plants revealed an increased expression of multiple ABA-responsive genes in the mutants. Taken together, our results uncover TOPP1 and AtI-2 as negative regulators of ABA signaling.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.