Evidence map›Paper›PMID 26942219›Full record

ArticleBiophysics reports2015

Analysis of chromatin fibers in Hela cells with electron tomography.

Xiaomin Li, Hongli Feng, Jianguo Zhang, Lei Sun, Ping Zhu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysics reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Biophysics reports · 2018
    Article
  3. Cryo-electron microscopy of chromatin biology.Acta crystallographica. Section D, Structural biology · 2017
    Review
  4. Gene functioning and storage within a folded genome.Cellular & molecular biology letters · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Xiaomin Li *National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.
Hongli Feng *National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.
Jianguo ZhangCenter for Biological Imaging, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.
Lei SunCenter for Biological Imaging, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.
Ping ZhuNational Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The presence and folding pattern of chromatin in eukaryotic cells remain elusive and controversial. In this study, we prepared ultra-thin sections of Hela cells with three different fixation and sectioning methods, i.e., chemical fixation, high pressure freezing with freeze substitution, and cryo-ultramicrotomy with SEM-FIB (focused ion beam), and analyzed in vivo architecture of chromatin fibers in Hela nuclei with electron tomography technology. The results suggest that the chromatin fibers in eukaryotic Hela cells are likely organized in an architecture with a diameter of about 30 nm.

Indexed as

Chemical fixationChromatin fiberCryo-ultramicrotomyDualBeam-FIBElectron tomographyHela cellHigh-pressure freezing

Identifiers

PMID26942219
PMCPMC4762132

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.