ArticleTheranostics2016
Development of a Loop Mediated Isothermal Amplification (LAMP) - Surface Enhanced Raman spectroscopy (SERS) Assay for the Detection of Salmonella Enterica Serotype Enteritidis.
Article in Theranostics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Surface-Enhanced Raman Spectroscopy for Viral Diagnostics: Principles, Strategies, Clinical Challenges, and Future Directions.Chemical reviews · 2026Review
- Advancing Food Safety Surveillance: Rapid and Sensitive Biosensing Technologies for Foodborne Pathogenic Bacteria.Foods (Basel, Switzerland) · 2025Review
- Metal and metal oxide nanoparticle-assisted molecular assays for the detection of Salmonella.Discover nano · 2025Review
- Prevalence of pathogens associated with neonatal gastrointestinal infections: a systematic review and meta-analysis.Gut pathogens · 2025Review
- Research progress of loop-mediated isothermal amplification in the detection of Salmonella for food safety applications.Discover nano · 2024Review
- Facial Preparation of Cyclometalated Iridium (III) Nanowires as Highly Efficient Electrochemiluminescence Luminophores for Biosensing.Biosensors · 2023Article
- LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection.Biosensors · 2022Review
- Principles and Applications of Loop-Mediated Isothermal Amplification to Point-of-Care Tests.Biosensors · 2022Review
- Rapid Detection of Virus Nucleic Acid via Isothermal Amplification on Plasmonic Enhanced Digitizing Biosensor.Biosensors · 2022Article
- Visible-light and near-infrared fluorescence and surface-enhanced Raman scattering point-of-care sensing and bio-imaging: a review.Chemical Society reviews · 2022Review
- Recent Advances on Functional Nucleic-Acid Biosensors.Sensors (Basel, Switzerland) · 2021Review
- Review
- Development of Surface-Enhanced Raman Scattering (SERS)-Based Surface-Corrugated Nanopillars for Biomolecular Detection of Colorectal Cancer.Biosensors · 2020Article
- A rapid novel visualized loop-mediated isothermal amplification method for Salmonella detection targeting at fimW gene.Poultry science · 2020Article
- Multifuntional Gold Nanoparticles for the SERS Detection of Pathogens Combined with a LAMP-in-Microdroplets Approach.Materials (Basel, Switzerland) · 2020Article
- Development of a rapid, one-step-visual method to detectIranian journal of veterinary research · 2020Article
- Diagnosis of Bacterial Pathogens in the Urine of Urinary-Tract-Infection Patients Using Surface-Enhanced Raman Spectroscopy.Molecules (Basel, Switzerland) · 2018Article
- A disposable microcapsule array chip fabricated by ice printing combined with isothermal amplification for Salmonella DNA detection.RSC advances · 2018Article
- Analysis of Biomolecules Based on the Surface Enhanced Raman Spectroscopy.Nanomaterials (Basel, Switzerland) · 2018Review
- Hexagonal Boron Nitride Functionalized with Au Nanoparticles-Properties and Potential Biological Applications.Nanomaterials (Basel, Switzerland) · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
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Abstract
As a major foodborne pathogen, Salmonella enterica serotype Enteritidis is increasingly rising as a global health concern. Here, we developed an integrated assay that combines loop mediated isothermal amplification (LAMP) and surface enhanced Raman spectroscopy (SERS) for DNA detection of S. Enteritidis using specifically designed Raman active Au-nanoprobes. The target DNA was amplified by LAMP and then labeled with Au-nanoprobes comprised of gold nanoparticle-modified with specific cy5/DNA probes to allow the detection by SERS. The sensitivity of the developed LAMP-SERS detection assay (66 CFU/mL) was ~100-fold higher than the conventional polymerase chain reaction (PCR) method. Significantly, this technique allowed highly specific detection of the target DNA of S. Enteritidis and could differentiate it from the DNA of closely related bacterial species or non-specific contamination, making it more accurate and reliable than the standard LAMP technique. The applicability of detection of S. Enteritidis in milk samples using LAMP-SERS assay was validated as well. In sum, the developed LAMP-SERS assay is highly specific and sensitive, and has the potential to be applied for rapid detection of different foodborne pathogens and other microbial contaminants.
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