ArticleMolecular biology of the cell2016
Structural comparison of the Caenorhabditis elegans and human Ndc80 complexes bound to microtubules reveals distinct binding behavior.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Pathogenic KIF1A R350 mutations disrupt a conserved and conformation-dependent kinesin-tubulin salt bridge.Nature communications · 2026Article
- Cryo-EM unveils kinesin KIF1A's processivity mechanism and the impact of its pathogenic variant P305L.Nature communications · 2024Article
- Structural mechanism of outer kinetochore Dam1-Ndc80 complex assembly on microtubules.Science (New York, N.Y.) · 2023Article
- Nucleotide-free structures of KIF20A illuminate atypical mechanochemistry in this kinesin-6.Open biology · 2023Article
- Cytoskeletal regulation of a transcription factor by DNA mimicry via coiled-coil interactions.Nature cell biology · 2022Article
- Potential therapeutic targets of the nuclear division cycle 80 (NDC80) complexes genes in lung adenocarcinoma.Journal of Cancer · 2020Article
- Kinetochore Recruitment of the Spindle and Kinetochore-Associated (Ska) Complex Is Regulated by Centrosomal PP2A inGenetics · 2019Article
- Microtubule structure by cryo-EM: snapshots of dynamic instability.Essays in biochemistry · 2018Review
- Cryo-EM reveals the structural basis of microtubule depolymerization by kinesin-13s.Nature communications · 2018Article
- Structural and functional differences between porcine brain and budding yeast microtubules.Cell cycle (Georgetown, Tex.) · 2018Article
- Microtubule Tip Tracking by the Spindle and Kinetochore Protein Ska1 Requires Diverse Tubulin-Interacting Surfaces.Current biology : CB · 2017Article
- Challenges and opportunities in the high-resolution cryo-EM visualization of microtubules and their binding partners.Current opinion in structural biology · 2017Review
- Structural differences between yeast and mammalian microtubules revealed by cryo-EM.The Journal of cell biology · 2017Article
- Astrin-SKAP complex reconstitution reveals its kinetochore interaction with microtubule-bound Ndc80.eLife · 2017Article
- Purification and characterisation of the fission yeast Ndc80 complex.Protein expression and purification · 2017Article
- Near-atomic cryo-EM structure of PRC1 bound to the microtubule.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
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3 authors.
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Abstract
During cell division, kinetochores must remain tethered to the plus ends of dynamic microtubule polymers. However, the molecular basis for robust kinetochore-microtubule interactions remains poorly understood. The conserved four-subunit Ndc80 complex plays an essential and direct role in generating dynamic kinetochore-microtubule attachments. Here we compare the binding of theCaenorhabditis elegansand human Ndc80 complexes to microtubules at high resolution using cryo-electron microscopy reconstructions. Despite the conserved roles of the Ndc80 complex in diverse organisms, we find that the attachment mode of these complexes for microtubules is distinct. The human Ndc80 complex binds every tubulin monomer along the microtubule protofilament, whereas theC. elegansNdc80 complex binds more tightly to β-tubulin. In addition, theC. elegansNdc80 complex tilts more toward the adjacent protofilament. These structural differences in the Ndc80 complex between different species may play significant roles in the nature of kinetochore-microtubule interactions.
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