ArticleNucleic acids research2016
Engineering a ribozyme cleavage-induced split fluorescent aptamer complementation assay.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
- A hammerhead ribozyme selects mechanically stable conformations for catalysis against viral RNA.Communications biology · 2025Article
- Toehold-mediated strand displacement to measure released product from self-cleaving ribozymes.RNA (New York, N.Y.) · 2022Article
- Strategies for Improving Small-Molecule Biosensors in Bacteria.Biosensors · 2022Review
- Digital Microfluidics Chips for the Execution and Real-Time Monitoring of Multiple Ribozymatic Cleavage Reactions.ACS omega · 2021Article
- Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors.Nucleic acids research · 2020Review
- From fluorescent proteins to fluorogenic RNAs: Tools for imaging cellular macromolecules.Protein science : a publication of the Protein Society · 2019Review
- Self-powered RNA nanomachine driven by metastable structure.Nucleic acids research · 2019Article
- Intracellular Imaging with Genetically Encoded RNA-based Molecular Sensors.Nanomaterials (Basel, Switzerland) · 2019Review
- Isothermal folding of a light-up bio-orthogonal RNA origami nanoribbon.Scientific reports · 2018Article
- Light-Up RNA Aptamers and Their Cognate Fluorogens: From Their Development to Their Applications.International journal of molecular sciences · 2017Review
- A Fluorescent Split Aptamer for Visualizing RNA-RNA Assembly In Vivo.ACS synthetic biology · 2017Article
- Use of Baby Spinach and Broccoli for imaging of structured cellular RNAs.Nucleic acids research · 2017Article
- Illuminating Messengers: An Update and Outlook on RNA Visualization in Bacteria.Frontiers in microbiology · 2017Review
- Split Spinach Aptamer for Highly Selective Recognition of DNA and RNA at Ambient Temperatures.Chembiochem : a European journal of chemical biology · 2016Article
- RNA Fluorescence with Light-Up Aptamers.Frontiers in chemistry · 2016Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Hammerhead ribozymes are self-cleaving RNA molecules capable of regulating gene expression in living cells. Their cleavage performance is strongly influenced by intra-molecular loop-loop interactions, a feature not readily accessible through modern prediction algorithms. Ribozyme engineering and efficient implementation of ribozyme-based genetic switches requires detailed knowledge of individual self-cleavage performances. By rational design, we devised fluorescent aptamer-ribozyme RNA architectures that allow for the real-time measurement of ribozyme self-cleavage activity in vitro The engineered nucleic acid molecules implement a split Spinach aptamer sequence that is made accessible for strand displacement upon ribozyme self-cleavage, thereby complementing the fluorescent Spinach aptamer. This fully RNA-based ribozyme performance assay correlates ribozyme cleavage activity with Spinach fluorescence to provide a rapid and straightforward technology for the validation of loop-loop interactions in hammerhead ribozymes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.