ArticlePloS one2016
Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS Imaging.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Are all dietary fibers equal for patients with inflammatory bowel disease? A systematic review of randomized controlled trials.Nutrition reviews · 2022Pooled it
- Optimization of Selenium Fortification and Ultrasound-Assisted Extraction of Bioactive Selenium-Polysaccharide Fractions from Germinated Oats (Foods (Basel, Switzerland) · 2026Article
- Germination as a Strategy to Enhance the Bioactivity of Whole Grains: Phytochemical Remodeling and Health Implications.Comprehensive reviews in food science and food safety · 2026Review
- The Metabolic Awakening of Barley Seedlings: A Time-Resolved View of How Germination and Light Shape Tissue-Specific Metabolic Networks.Plant direct · 2026Article
- Influence of oven-drying pre-treatment on the metabolic profile of extracts from brewer's spent grains.Frontiers in nutrition · 2026Article
- Integrated metabolomic and transcriptomic strategies to reveal adaptive mechanisms in barley plant during germination stage under waterlogging stress.Journal of plant research · 2025Article
- Spatial specificity of metabolism regulation of abscisic acid-imposed seed germination inhibition in Korean pine (Pinus koraiensis sieb et zucc).Frontiers in plant science · 2024Article
- Plant Life with and without Oxygen: A Metabolomics Approach.International journal of molecular sciences · 2023Review
- Optimization of Mass Spectrometry Imaging for Drug Metabolism and Distribution Studies in the Zebrafish Larvae Model: A Case Study with the Opioid Antagonist Naloxone.International journal of molecular sciences · 2023Article
- MALDI MS Imaging of Cucumbers.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Genome-Wide Association Study of Salt Tolerance-Related Traits during Germination and Seedling Development in anInternational journal of molecular sciences · 2022Article
- Mutations inInternational journal of molecular sciences · 2022Article
- Article
- Comparative Metabolomics Reveals Two Metabolic Modules Affecting Seed Germination in Rice (Metabolites · 2021Article
- Detection and Verification of QTL for Salinity Tolerance at Germination and Seedling Stages Using Wild Barley Introgression Lines.Plants (Basel, Switzerland) · 2021Article
- Phosphorus deprivation affects composition and spatial distribution of membrane lipids in legume nodules.Plant physiology · 2021Article
- Spatially Resolved Mass Spectrometry at the Single Cell: Recent Innovations in Proteomics and Metabolomics.Journal of the American Society for Mass Spectrometry · 2021Review
- Plant Single-Cell Metabolomics-Challenges and Perspectives.International journal of molecular sciences · 2020Review
- Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging of Metabolites during Sorghum Germination.Plant physiology · 2020Article
- Application of Mass Spectrometry Imaging for Visualizing Food Components.Foods (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MALDI mass spectrometry imaging was performed to localize metabolites during the first seven days of the barley germination. Up to 100 mass signals were detected of which 85 signals were identified as 48 different metabolites with highly tissue-specific localizations. Oligosaccharides were observed in the endosperm and in parts of the developed embryo. Lipids in the endosperm co-localized in dependency on their fatty acid compositions with changes in the distributions of diacyl phosphatidylcholines during germination. 26 potentially antifungal hordatines were detected in the embryo with tissue-specific localizations of their glycosylated, hydroxylated, and O-methylated derivates. In order to reveal spatio-temporal patterns in local metabolite compositions, multiple MSI data sets from a time series were analyzed in one batch. This requires a new preprocessing strategy to achieve comparability between data sets as well as a new strategy for unsupervised clustering. The resulting spatial segmentation for each time point sample is visualized in an interactive cluster map and enables simultaneous interactive exploration of all time points. Using this new analysis approach and visualization tool germination-dependent developments of metabolite patterns with single MS position accuracy were discovered. This is the first study that presents metabolite profiling of a cereals' germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents. Their detailed localization as well as the MS cluster analyses for on-tissue metabolite profile mapping revealed important information for the understanding of the germination process, which is of high scientific interest.
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