ArticleJournal of the American Chemical Society2016
A Programmable DNA Origami Platform to Organize SNAREs for Membrane Fusion.
Article in Journal of the American Chemical Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed.
- Vesicle-Templated Self-Assembly of Programmable Freestanding Multi-μm DNA Shells.Nano letters · 2026Article
- DNA-Assisted Assays for Studying Lipid Transfer Between Membranes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Facile Splint-Free Circularization of ssDNA with T4 DNA Ligase by Redesigning the Linear Substrate to Form an Intramolecular Dynamic Nick.Biomolecules · 2024Article
- Double-Transmembrane Domain of SNAREs Decelerates the Fusion by Increasing the Protein-Lipid Mismatch.Journal of molecular biology · 2023Article
- Recent Advances in DNA Origami-Engineered Nanomaterials and Applications.Chemical reviews · 2023Review
- Digital nanoreactors to control absolute stoichiometry and spatiotemporal behavior of DNA receptors within lipid bilayers.Nature communications · 2023Article
- Gene-encoding DNA origami for mammalian cell expression.Nature communications · 2023Article
- Functionalizing DNA origami to investigate and interact with biological systems.Nature reviews. Materials · 2023Article
- The beginning and the end of SNARE-induced membrane fusion.FEBS open bio · 2022Review
- Single-particle combinatorial multiplexed liposome fusion mediated by DNA.Nature chemistry · 2022Article
- Membrane-Interacting DNA Nanotubes Induce Cancer Cell Death.Nanomaterials (Basel, Switzerland) · 2021Article
- Sorting sub-150-nm liposomes of distinct sizes by DNA-brick-assisted centrifugation.Nature chemistry · 2021Article
- DNA Nanodevices to Probe and Program Membrane Organization, Dynamics, and Applications.The Journal of membrane biology · 2020Review
- Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics.APL bioengineering · 2020Review
- Programmable Nanodisc Patterning by DNA Origami.Nano letters · 2020Article
- Synergistic roles of Synaptotagmin-1 and complexin in calcium-regulated neuronal exocytosis.eLife · 2020Article
- Proximity-induced caspase-9 activation on a DNA origami-based synthetic apoptosome.Nature catalysis · 2020Article
- Engineering Lipid Membranes with Programmable DNA Nanostructures.Advanced biosystems · 2020Review
- The Fusion of Lipid and DNA Nanotechnology.Genes · 2019Review
- Modifying Membrane Morphology and Interactions with DNA Origami Clathrin-Mimic Networks.ACS nano · 2019Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes are the core molecular machinery of membrane fusion, a fundamental process that drives inter- and intracellular communication and trafficking. One of the questions that remains controversial has been whether and how SNAREs cooperate. Here we show the use of self-assembled DNA-nanostructure rings to template uniform-sized small unilamellar vesicles containing predetermined maximal number of externally facing SNAREs to study the membrane-fusion process. We also incorporated lipid-conjugated complementary ssDNA as tethers into vesicle and target membranes, which enabled bypass of the rate-limiting docking step of fusion reactions and allowed direct observation of individual membrane-fusion events at SNARE densities as low as one pair per vesicle. With this platform, we confirmed at the single event level that, after docking of the templated-SUVs to supported lipid bilayers (SBL), one to two pairs of SNAREs are sufficient to drive fast lipid mixing. Modularity and programmability of this platform makes it readily amenable to studying more complicated systems where auxiliary proteins are involved.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.