ArticleNature protocols2016
Assembly and operation of the autopatcher for automated intracellular neural recording in vivo.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 71 citations in OpenAlex.
- Artificial Gap Junctional Channel Inhibiting the Proliferation of Hepatocellular Carcinoma Cells.Angewandte Chemie (International ed. in English) · 2026Article
- A robotic patch-clamp system with real-time localization and phase-synchronized capture of dynamic in vivo cells using micropipette resistance modelling.Microsystems & nanoengineering · 2026Article
- A Scalable Fishbone Nanowire Array (FINE) for 3D Quasi-Intracellular Recording in Intact Brains.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections.eLife · 2024Article
- Synthetic biology in plants.Plant biotechnology (Tokyo, Japan) · 2024Article
- Patch-walking: Coordinated multi-pipette patch clamp for efficiently finding synaptic connections.bioRxiv : the preprint server for biology · 2024Article
- High-throughput genetic manipulation of multicellular organisms using a machine-vision guided embryonic microinjection robot.Genetics · 2024Article
- Neuron Contact Detection Based on Pipette Precise Positioning for Robotic Brain-Slice Patch Clamps.Sensors (Basel, Switzerland) · 2023Article
- Electrophysiological and morphological characterization of single neurons in intact human brain organoids.Journal of neuroscience methods · 2023Article
- Current approaches to characterize micro- and macroscale circuit mechanisms of Parkinson's disease in rodent models.Experimental neurology · 2022Review
- From End to End: Gaining, Sorting, and Employing High-Density Neural Single Unit Recordings.Frontiers in neuroinformatics · 2022Review
- Scaled, high fidelity electrophysiological, morphological, and transcriptomic cell characterization.eLife · 2021Article
- Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts.Bio-protocol · 2021Article
- [Communication sound recognition and response modification in the secondary auditory cortex of female mice].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2021Article
- Rapid Cortical Adaptation and the Role of Thalamic Synchrony during Wakefulness.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives.Sensors (Basel, Switzerland) · 2021Review
- Automatic deep learning-driven label-free image-guided patch clamp system.Nature communications · 2021Article
- Compensation of physiological motion enables high-yield whole-cell recording in vivo.Journal of neuroscience methods · 2021Article
- TbScience advances · 2021Article
- Single neuron recording: progress towards high-throughput analysis.Bioelectronics in medicine · 2020Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
Abstract
Whole-cell patch clamping in vivo is an important neuroscience technique that uniquely provides access to both suprathreshold spiking and subthreshold synaptic events of single neurons in the brain. This article describes how to set up and use the autopatcher, which is a robot for automatically obtaining high-yield and high-quality whole-cell patch clamp recordings in vivo. By following this protocol, a functional experimental rig for automated whole-cell patch clamping can be set up in 1 week. High-quality surgical preparation of mice takes ∼1 h, and each autopatching experiment can be carried out over periods lasting several hours. Autopatching should enable in vivo intracellular investigations to be accessible by a substantial number of neuroscience laboratories, and it enables labs that are already doing in vivo patch clamping to scale up their efforts by reducing training time for new lab members and increasing experimental durations by handling mentally intensive tasks automatically.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.