ArticleNature protocols2016
Interferometric scattering microscopy and its combination with single-molecule fluorescence imaging.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
70 citing papers in PubMed.
- Toward a unifying mechanistic model of eukaryotic translation initiation through integrative single-molecule, structural, and computational insights.Current opinion in structural biology · 2026Review
- Using rotational integration of oblique interferometric scattering to track axial spatiotemporal responses of tubular membrane protrusions.Nature communications · 2026Article
- Rapid trapping and label-free optical characterization of single nanoscale extracellular vesicles and nanoparticles in solution.Light, science & applications · 2026Article
- Visualizing Dynamic Processes in Energy Materials by Interferometric Scattering Microscopy.ACS energy letters · 2026Review
- Quantifying Non-Gaussian Diffusion in Transient Microscopy Using Excess Kurtosis.The journal of physical chemistry letters · 2026Article
- Review
- AI-assisted label-free single-particle analysis of milk-derived extracellular vesicles enabled by nanotweezers.Npj biosensing · 2026Article
- Deciphering Evolution, Function, and Observation of Crystallization-Driven Self-Assembly.Chemical reviews · 2025Review
- Article
- High-Speed Interferometric Scattering Tracking Microscopy of Compartmentalized Lipid Diffusion in Living Cells.Chemphyschem : a European journal of chemical physics and physical chemistry · 2025Article
- A correlative quantitative phase contrast and fluorescence super-resolution microscope for imaging molecules in their cellular context.bioRxiv : the preprint server for biology · 2025Article
- Enhanced Interferometric Imaging by Rotating Coherent Scattering Microscopy.ACS photonics · 2025Article
- Real-time label-free imaging of living crystallization-driven self-assembly.Nature communications · 2025Article
- Multispectral Interferometric Scattering Microscopy Imaging of Gold and Silver Nanoparticles.ACS applied nano materials · 2025Article
- Spatial light modulation for interferometric scattering microscopy.Journal of microscopy · 2025Article
- Label-Free Anti-Brownian Trapping of Single Nanoparticles in Solution.The journal of physical chemistry. C, Nanomaterials and interfaces · 2024Article
- In Situ and Label-Free Quantification of Membrane Protein-Ligand Interactions Using Optical Imaging Techniques: A Review.Biosensors · 2024Review
- Size photometry and fluorescence imaging of immobilized immersed extracellular vesicles.Journal of extracellular vesicles · 2024Article
- Non-invasive estimation of the powder size distribution from a single speckle image.Light, science & applications · 2024Article
- Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interferometric scattering microscopy (iSCAT) is a light scattering-based imaging modality that offers a unique combination of imaging speed and precision for tracking nanoscopic labels and enables label-free optical sensing down to the single-molecule level. In contrast to fluorescence, iSCAT does not suffer from limitations associated with dye photochemistry and photophysics, or the requirement for fluorescent labeling. Here we present a protocol for constructing an iSCAT microscope from commercially available optical components and demonstrate its compatibility with simultaneously operating single-molecule, objective-type, total internal reflection fluorescence microscopy. Given an intermediate level of experience with optics and microscopy, for instance graduate-level familiarity with laser beam steering and optical components, this protocol can be completed in a time frame of 2 weeks.
Indexed as
Identifiers
26938114What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.