ArticlePloS one2016
Reproducibility of Fluorescent Expression from Engineered Biological Constructs in E. coli.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Host-aware Identification of Intrinsic Gene Expression Biopart Parameters using Combinatorial Libraries.Nature communications · 2026Article
- The adaptive landscapes of three globaleLife · 2026Article
- qByte: An open-source isothermal fluorimeter for democratizing analysis of nucleic acids, proteins and cells.PLoS biology · 2025Article
- The highly rugged yet navigable regulatory landscape of the bacterial transcription factor TetR.Nature communications · 2024Article
- Measuring the burden of hundreds of BioBricks defines an evolutionary limit on constructability in synthetic biology.Nature communications · 2024Article
- Measuring the burden of hundreds of BioBricks defines an evolutionary limit on constructability in synthetic biology.bioRxiv : the preprint server for biology · 2024Article
- Interlaboratory Reproducibility in Growth and Reporter Expression in the CyanobacteriumACS synthetic biology · 2023Article
- Advancing reproducibility can ease the 'hard truths' of synthetic biology.Synthetic biology (Oxford, England) · 2023Review
- Comparative analysis of three studies measuring fluorescence from engineered bacterial genetic constructs.PloS one · 2021Article
- Article
- Biological Materials: The Next Frontier for Cell-Free Synthetic Biology.Frontiers in bioengineering and biotechnology · 2020Review
- Telescope: an interactive tool for managing large-scale analysis from mobile devices.GigaScience · 2020Article
- Absolute quantification of translational regulation and burden using combined sequencing approaches.Molecular systems biology · 2019Article
- Tuning dCas9's ability to block transcription enables robust, noiseless knockdown of bacterial genes.Molecular systems biology · 2018Article
- A minimum information standard for reproducing bench-scale bacterial cell growth and productivity.Communications biology · 2018Article
- A Cas9-based toolkit to program gene expression in Saccharomyces cerevisiae.Nucleic acids research · 2017Article
- FlowCal: A User-Friendly, Open Source Software Tool for Automatically Converting Flow Cytometry Data from Arbitrary to Calibrated Units.ACS synthetic biology · 2016Article
- A synthetic biology standard for Chinese Hamster Ovary cell genome monitoring and contaminant detection by polymerase chain reaction.SpringerPlus · 2016Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions around the world measured fluorescence from three engineered constitutive constructs in E. coli. Few participants were able to measure absolute fluorescence, so data was analyzed in terms of ratios. Precision was strongly related to fluorescent strength, ranging from 1.54-fold standard deviation for the ratio between strong promoters to 5.75-fold for the ratio between the strongest and weakest promoter, and while host strain did not affect expression ratios, choice of instrument did. This result shows that high quantitative precision and reproducibility of results is possible, while at the same time indicating areas needing improved laboratory practices.
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