ArticleEndocrinology2016
Ablation of KNDy Neurons Results in Hypogonadotropic Hypogonadism and Amplifies the Steroid-Induced LH Surge in Female Rats.
Article in Endocrinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 73 citations in OpenAlex.
- Critical role of arcuate nucleus kisspeptin and Kiss1R in regulation of the ovine luteinizing hormone surge.Journal of neuroendocrinology · 2025Article
- Article
- Molecular pathways affecting reproductive efficiency in seasonal breeders: prospects and implications for improving fertility in donkeys.Frontiers in veterinary science · 2025Review
- Selective depletion of kisspeptin neurons in the hypothalamic arcuate nucleus in early juvenile life reduces pubertal LH secretion and delays puberty onset in mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- History of the Development of Knowledge about the Neuroendocrine Control of Ovulation-Recent Knowledge on the Molecular Background.International journal of molecular sciences · 2024Review
- Review
- Article
- Hypothalamic kisspeptin neurons as potential mediators of estradiol negative and positive feedback.Peptides · 2023Review
- Serotonin stimulates female preoptic area kisspeptin neuronsFrontiers in endocrinology · 2023Article
- Definition of the estrogen negative feedback pathway controlling the GnRH pulse generator in female mice.Nature communications · 2022Article
- Sodium-glucose cotransporter 2 inhibitor ameliorates high fat diet-induced hypothalamic-pituitary-ovarian axis disorders.The Journal of physiology · 2022Review
- Conditional Deletion of KOR (Oprk1) in Kisspeptin Cells Does Not Alter LH Pulses, Puberty, or Fertility in Mice.Endocrinology · 2022Article
- Neuroendocrine control of gonadotropin-releasing hormone: Pulsatile and surge modes of secretion.Journal of neuroendocrinology · 2022Review
- Sexual Dimorphism in Kisspeptin Signaling.Cells · 2022Review
- In vivo imaging of the GnRH pulse generator reveals a temporal order of neuronal activation and synchronization during each pulse.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Optogenetic stimulation of Kiss1Frontiers in endocrinology · 2022Article
- Neuroendocrine mechanisms underlying estrogen positive feedback and the LH surge.Frontiers in neuroscience · 2022Review
- Tachykinins, new players in the control of reproduction and food intake: A comparative review in mammals and teleosts.Frontiers in endocrinology · 2022Review
- Article
- Makorin rings the kisspeptin bell to signal pubertal initiation.The Journal of clinical investigation · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In the human infundibular (arcuate) nucleus, a subpopulation of neurons coexpress kisspeptin and neurokinin B (NKB), 2 peptides required for normal reproductive function. A homologous group of neurons exists in the arcuate nucleus of rodents, termed KNDy neurons based on the coexpression of kisspeptin, NKB, and dynorphin. To study their function, we recently developed a method to selectively ablate KNDy neurons using NK3-SAP, a neurokinin 3 receptor agonist conjugated to saporin (SAP). Here, we ablated KNDy neurons in female rats to determine whether these neurons are required for estrous cyclicity and the steroid induced LH surge. NK3-SAP or Blank-SAP (control) was microinjected into the arcuate nucleus using stereotaxic surgery. After monitoring vaginal smears for 3-4 weeks, rats were ovariectomized and given 17β-estradiol and progesterone in a regimen that induced an afternoon LH surge. Rats were killed at the time of peak LH levels, and brains were harvested for NKB and dual labeled GnRH/Fos immunohistochemistry. In ovary-intact rats, ablation of KNDy neurons resulted in hypogonadotropic hypogonadism, characterized by low levels of serum LH, constant diestrus, ovarian atrophy with increased follicular atresia, and uterine atrophy. Surprisingly, the 17β-estradiol and progesterone-induced LH surge was 3 times higher in KNDy-ablated rats. Despite the marked increase in the magnitude of the LH surge, the number of GnRH or anterior ventral periventricular nucleus neurons expressing Fos was not significantly different between groups. Our studies show that KNDy neurons are essential for tonic levels of serum LH and estrous cyclicity and may play a role in limiting the magnitude of the LH surge.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.