ArticleJournal of biological methods
Cell-based assays using calcein acetoxymethyl ester show variation in fluorescence with treatment conditions.
Article in Journal of biological methods. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
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- Characterization of the anticancer effect of mebendazole and its interaction with standard cytotoxic drugs in patient tumor cellsOncology reports · 2026Article
- The effects of house dust-derived mixtures of organophosphate esters on Leydig cell phenotype, function, and lipidome†.Biology of reproduction · 2025Article
- From Medical Imaging to Bioprinted Tissues: The Importance of Workflow Optimisation for Improved Cell Function.Expert reviews in molecular medicine · 2025Review
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- The extracellular vesicle proteomes of Sorghum bicolor and Arabidopsis thaliana are partially conserved.Plant physiology · 2024Article
- Methods in cancer research: Assessing therapy response of spheroid cultures by life cell imaging using a cost-effective live-dead staining protocol.Biology methods & protocols · 2024Article
- Chitosan nanoparticle toxicity: A comprehensive literature review ofToxicology reports · 2023Review
- spinDrop: a droplet microfluidic platform to maximise single-cell sequencing information content.Nature communications · 2023Article
- A designed cyclic analogue of gomesin has potent activity against Staphylococcus aureus biofilms.The Journal of antimicrobial chemotherapy · 2022Article
- The Effects of Organophosphate Esters Used as Flame Retardants and Plasticizers on Granulosa, Leydig, and Spermatogonial Cells Analyzed Using High-Content Imaging.Toxicological sciences : an official journal of the Society of Toxicology · 2022Article
- A Microfluidic Multisize Spheroid Array for Multiparametric Screening of Anticancer Drugs and Blood-Brain Barrier Transport Properties.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- In Vitro Liver Toxicity Testing of Chemicals: A Pragmatic Approach.International journal of molecular sciences · 2021Review
- Use of in vitro bone models to screen for altered bone metabolism, osteopathies, and fracture healing: challenges of complex models.Archives of toxicology · 2020Review
- Decorin Protects Cardiac Myocytes against Simulated Ischemia/Reperfusion Injury.Molecules (Basel, Switzerland) · 2020Article
- Cytotoxic potentials of silibinin assisted silver nanoparticles on human colorectal HT-29 cancer cells.Bioinformation · 2020Article
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The use of fluorogenic compounds in cell and molecular biology has increased in both frequency and range of applications. However, such compounds may introduce artifacts in intracellular fluorescence and cell number estimations as a consequence of interaction with exogenous stimulants, necessitating the use of adequate controls for accurate measurements and valid conclusions. Using calcein acetoxymethyl ester (AM) in combination with various exogenous cellular treatments, we report that the standard practice of direct normalization of experimental values to controls is insufficient for fluorogenic measurements. Treatments applied to cells may influence intracellular conversion of the fluorogenic compound, thereby enhancing or decreasing fluorescence relative to controls. We hereby encourage caution and recommend normalization of cellular fluorescence within each treatment group before comparison to controls.
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Registered trials
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