ArticleF1000Research2015
A DNA extraction protocol for improved DNA yield from individual mosquitoes.
Article in F1000Research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Host preference and resting behavior of Anopheles coluzzii (Diptera: Culicidae) on the island of São Tomé, West Africa.Journal of medical entomology · 2026Article
- Insecticide resistance status predetermines behavioural responses and oviposition fitness in Anopheles coluzzii.PloS one · 2026Article
- Genetics of range expansion and admixture of Aedes aegypti populations in California.BMC genomics · 2025Article
- Polymeric Anti-Antibiotic Microparticles to Prevent Antibiotic Resistance Evolution.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Defining the genetics of the widely used G3 strain of the mosquito, Anopheles gambiae.Scientific reports · 2025Article
- Article
- Semi-field experiments reveal contrasted predation and movement patterns of aquatic macroinvertebrate predators of Anopheles gambiae larvae.Malaria journal · 2025Article
- Evaluation of Protocols for DNA Extraction from IndividualMethods and protocols · 2024Article
- Cas9/guide RNA-based gene-drive dynamics following introduction and introgression into diverse anopheline mosquito genetic backgrounds.BMC genomics · 2024Article
- Patterns of Gene Flow inEvolutionary applications · 2024Article
- Anopheles gambiae on remote islands in the Indian Ocean: origins and prospects for malaria elimination by genetic modification of extant populations.Scientific reports · 2023Article
- Genomic signatures of local adaptation in recent invasive Aedes aegypti populations in California.BMC genomics · 2023Article
- Population Genetics ofInsects · 2022Article
- Spontaneous mutation rate estimates for the principal malaria vectors Anopheles coluzzii and Anopheles stephensi.Scientific reports · 2022Article
- Selection of sites for field trials of genetically engineered mosquitoes with gene drive.Evolutionary applications · 2021Article
- The origin of island populations of the African malaria mosquito, Anopheles coluzzii.Communications biology · 2021Article
- Complete mitogenome sequence ofMitochondrial DNA. Part B, Resources · 2020Article
- Article
- Introgression between Anopheles gambiae and Anopheles coluzzii in Burkina Faso and its associations with kdr resistance and Plasmodium infection.Malaria journal · 2019Article
- Genome-wide divergence among invasive populations of Aedes aegypti in California.BMC genomics · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Typical DNA extraction protocols from commercially available kits provide an adequate amount of DNA from a single individual mosquito sufficient for PCR-based assays. However, next-generation sequencing applications and high-throughput SNP genotyping assays exposed the limitation of DNA quantity one usually gets from a single individual mosquito. Whole genome amplification could alleviate the issue but it also creates bias in genome representation. While trying to find alternative DNA extraction protocols for improved DNA yield, we found that a combination of the tissue lysis protocol from Life Technologies and the DNA extraction protocol from Qiagen yielded a higher DNA amount than the protocol using the Qiagen or Life Technologies kit only. We have not rigorously tested all the possible combinations of extraction protocols; we also only tested this on mosquito samples. Therefore, our finding should be noted as a suggestion for improving people's own DNA extraction protocols and not as an advertisement of a commercially available product.
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