ArticleProceedings of the National Academy of Sciences of the United States of America2016
Blocking rapid ice crystal growth through nonbasal plane adsorption of antifreeze proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 185 citations in OpenAlex.
- Rational engineering of an antifreeze-based biomaterial with dual-enhanced ice-binding and delivery for high-efficiency cryopreservation.Journal of advanced research · 2026Article
- Antifreeze Protein for Freeze-Thaw Durability Enhancement of Cement Mortar: Effects and Action Analysis.Materials (Basel, Switzerland) · 2026Article
- Discovering naturally occurring antifreeze peptides from microbiome by integrating protein language models and molecular dynamics simulation.Journal of materials chemistry. B · 2026Article
- Article
- An Ultrapotent, Ultraeconomical, Antifreeze Polypeptide.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Inhibition of ice recrystallization with designed twistless helical repeat proteins.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- How to measure the activity of antifreeze proteins?Biophysical reviews · 2025Review
- Atomistic Insights into the Chain-Length-Dependent Antifreeze Activity of Oligoprolines.Biomacromolecules · 2025Article
- BERT-DomainAFP: Antifreeze protein recognition and classification model based on BERT and structural domain annotation.iScience · 2025Article
- Tartronic Acid as a Potential Inhibitor of Pathological Calcium Oxalate Crystallization.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Extended Temperature Range of the Ice-Binding Protein Activity.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Preservation of human heart valves for replacement in children with heart valve disease: past, present and future.Cell and tissue banking · 2024Review
- The cryoprotective effect ofCurrent research in food science · 2024Article
- The Nanoliter Osmometer: Thermal Hysteresis Measurement.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Biomimetic Antibacterial Gelatin Hydrogels with Multifunctional Properties for Biomedical Applications.ACS applied materials & interfaces · 2023Article
- De novo designed ice-binding proteins from twist-constrained helices.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Nanoscopy of single antifreeze proteins reveals that reversible ice binding is sufficient for ice recrystallization inhibition but not thermal hysteresis.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Ice Recrystallization Inhibition Is Insufficient to Explain Cryopreservation Abilities of Antifreeze Proteins.Biomacromolecules · 2022Article
- Ice Recrystallization Inhibition by Amino Acids: The Curious Case of Alpha- and Beta-Alanine.The journal of physical chemistry letters · 2022Article
- Inhibition of Ice Recrystallization by Nanotube-Forming Cyclic Peptides.Biomacromolecules · 2022Article
Corrections and comments
- Commented on by
Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antifreeze proteins (AFPs) are a unique class of proteins that bind to growing ice crystal surfaces and arrest further ice growth. AFPs have gained a large interest for their use in antifreeze formulations for water-based materials, such as foods, waterborne paints, and organ transplants. Instead of commonly used colligative antifreezes such as salts and alcohols, the advantage of using AFPs as an additive is that they do not alter the physicochemical properties of the water-based material. Here, we report the first comprehensive evaluation of thermal hysteresis (TH) and ice recrystallization inhibition (IRI) activity of all major classes of AFPs using cryoscopy, sonocrystallization, and recrystallization assays. The results show that TH activities determined by cryoscopy and sonocrystallization differ markedly, and that TH and IRI activities are not correlated. The absence of a distinct correlation in antifreeze activity points to a mechanistic difference in ice growth inhibition by the different classes of AFPs: blocking fast ice growth requires rapid nonbasal plane adsorption, whereas basal plane adsorption is only relevant at long annealing times and at small undercooling. These findings clearly demonstrate that biomimetic analogs of antifreeze (glyco)proteins should be tailored to the specific requirements of the targeted application.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.