ArticleBMC nephrology2016
Novel methods for microCT-based analyses of vasculature in the renal cortex reveal a loss of perfusable arterioles and glomeruli in eNOS-/- mice.
Article in BMC nephrology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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26 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Preclinical techniques to investigate exercise training in vascular pathophysiology.American journal of physiology. Heart and circulatory physiology · 2021Pooled it
- Mapping the arterial vascular network in an intact human kidney using hierarchical phase-contrast tomography.Npj imaging · 2025Article
- Micro-computed tomography to visualize preserved vascular architecture in decellularized human vaginal tissue: explorative study.Scientific reports · 2025Article
- Development of novel technology for the visualization and quantitation of angiogenesis and the alveolar-capillary network in a mouse model of fibrosis.American journal of physiology. Lung cellular and molecular physiology · 2025Article
- Article
- Endothelial eNOS deficiency causes podocyte injury through NFAT2 and heparanase in diabetic mice.Scientific reports · 2024Article
- Mapping the blood vasculature in an intact human kidney using hierarchical phase-contrast tomography.bioRxiv : the preprint server for biology · 2024Article
- μCT imaging of a multi-organ vascular fingerprint in rats.PloS one · 2024Article
- A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution.Cell reports methods · 2023Article
- Endothelial Nitric Oxide Synthase (eNOS) and the Cardiovascular System: in Physiology and in Disease States.American journal of biomedical science & research · 2022Article
- New imaging tools to measure nephron numberJournal of developmental origins of health and disease · 2021Review
- Vessel network extraction and analysis of mouse pulmonary vasculature via X-ray micro-computed tomographic imaging.PLoS computational biology · 2021Article
- A Review of Ex Vivo X-ray Microfocus Computed Tomography-Based Characterization of the Cardiovascular System.International journal of molecular sciences · 2021Review
- Innovative high-resolution microCT imaging of animal brain vasculature.Brain structure & function · 2020Article
- Super-Resolution Imaging with Ultrasound for Visualization of the Renal Microvasculature in Rats Before and After Renal Ischemia: A Pilot Study.Diagnostics (Basel, Switzerland) · 2020Article
- Adipose derived mesenchymal stem cells seeded onto a decellularized nerve allograft enhances angiogenesis in a rat sciatic nerve defect model.Microsurgery · 2020Article
- Vascular Casting of Adult and Early Postnatal Mouse Lungs for Micro-CT Imaging.Journal of visualized experiments : JoVE · 2020Article
- New methods for objective angiogenesis evaluation of rat nerves using microcomputed tomography scanning and conventional photography.Microsurgery · 2020Article
- Photoacoustic Imaging of Nanoparticle Transport in the Kidneys at High Temporal Resolution.Angewandte Chemie (International ed. in English) · 2019Article
- Visualization of the intrarenal distribution of capillary blood flow.Physiological reports · 2019Article
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7 authors at 3 institutions in 2 countries.
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Abstract
backgroundTwo-dimensional measures of vascular architecture provide incomplete information about vascular structure. This study applied a novel rigorous method for 3D microCT-based analysis of total and cortical renal vasculature combined with a novel method to isolate and quantify the number of perfused glomeruli to assess vascular changes in eNOS-/- mice.
methodsTwo month old male wildtype and eNOS-/- mice were perfused with heparinized saline followed by radiopaque Microfil. The Microfil-perfused vasculature of excised kidneys was imaged by μCT with an isotropic voxel-size of 5.0 μm. For analysis of renal cortical vasculature, a custom algorithm was created to define the cortical volume of interest (VOI) as the entire volume within 600 μm of the renal surface. Vessel thickness in the whole kidney or renal cortex was analyzed by plotting the distribution of vascular volume at each measured thickness and examining differences between the genotypes at individual thicknesses. A second image processing algorithm was created to isolate, identify, and extract contrast perfused glomeruli from the cortical vessels.
resultsFractional vascular volume (vascular volume/kidney volume; VV/KV) and Vessel Number/mm (V.N) were significantly lower in eNOS-/- mice vs. WT (p < 0.05). eNOS-/- kidneys had significantly fewer perfusable vessels vs. WT in the range of 20-40 μm in thickness. The cortex of eNOS-/- kidneys had significantly lower VV, VV/cortical volume, and V.N, with an increase in the distance between vessels (all p < 0.05). The total volume of vessels in the range of 20-30 μm was significantly lower in the cortex of eNOS-/- mice compared to WT (p < 0.05). Moreover, the total number of perfused glomeruli was significantly decreased in eNOS-/- mice (p < 0.01).
conclusionsThe methods presented here demonstrate a new method to analyze contrast enhanced μCT images for vascular phenotyping of the murine kidney. These data also demonstrate that kidneys in eNOS-/- mice have severe defects in vascular perfusion/structure in the renal cortex.
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