ArticleCellular microbiology2016
Active and adaptive Legionella CRISPR-Cas reveals a recurrent challenge to the pathogen.
Article in Cellular microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 37 citations in OpenAlex.
- Genomic characterization ofPathogens and global health · 2026Article
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- Expanded geographic distribution for twomSphere · 2024Article
- Legionnaires' Disease in China Caused byMicroorganisms · 2023Article
- Article
- Legionella pneumophila Cas2 Promotes the Expression of Small Heat Shock Protein C2 That Is Required for Thermal Tolerance and Optimal Intracellular Infection.Infection and immunity · 2022Article
- Potential probiotic approaches to control Legionella in engineered aquatic ecosystems.FEMS microbiology ecology · 2022Review
- Highly efficient genome editing in Xanthomonas oryzae pv. oryzae through repurposing the endogenous type I-C CRISPR-Cas system.Molecular plant pathology · 2022Article
- Legionella pneumophila CRISPR-Cas Suggests Recurrent Encounters with One or More Phages in the FamilyApplied and environmental microbiology · 2021Article
- A multiplex CRISPR interference tool for virulence gene interrogation in Legionella pneumophila.Communications biology · 2021Article
- Pruning and Tending Immune Memories: Spacer Dynamics in the CRISPR Array.Frontiers in microbiology · 2021Review
- Type I-F CRISPR-Cas Distribution and Array Dynamics inG3 (Bethesda, Md.) · 2020Article
- CRISPR-Cas in Streptococcus pyogenes.RNA biology · 2019Review
- CRISPR-Cas systems are present predominantly on mobile genetic elements in Vibrio species.BMC genomics · 2019Article
- Cas4 Facilitates PAM-Compatible Spacer Selection during CRISPR Adaptation.Cell reports · 2018Article
- Article
- A Supervised Statistical Learning Approach for Accurate Legionella pneumophila Source Attribution during Outbreaks.Applied and environmental microbiology · 2017Article
- Priming in a permissive type I-C CRISPR-Cas system reveals distinct dynamics of spacer acquisition and loss.RNA (New York, N.Y.) · 2017Article
- Spacer capture and integration by a type I-F Cas1-Cas2-3 CRISPR adaptation complex.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clustered regularly interspaced short palindromic repeats with CRISPR-associated gene (CRISPR-Cas) systems are widely recognized as critical genome defense systems that protect microbes from external threats such as bacteriophage infection. Several isolates of the intracellular pathogen Legionella pneumophila possess multiple CRISPR-Cas systems (type I-C, type I-F and type II-B), yet the targets of these systems remain unknown. With the recent observation that at least one of these systems (II-B) plays a non-canonical role in supporting intracellular replication, the possibility remained that these systems are vestigial genome defense systems co-opted for other purposes. Our data indicate that this is not the case. Using an established plasmid transformation assay, we demonstrate that type I-C, I-F and II-B CRISPR-Cas provide protection against spacer targets. We observe efficient laboratory acquisition of new spacers under 'priming' conditions, in which initially incomplete target elimination leads to the generation of new spacers and ultimate loss of the invasive DNA. Critically, we identify the first known target of L. pneumophila CRISPR-Cas: a 30 kb episome of unknown function whose interbacterial transfer is guarded against by CRISPR-Cas. We provide evidence that the element can subvert CRISPR-Cas by mutating its targeted sequences - but that primed spacer acquisition may limit this mechanism of escape. Rather than generally impinging on bacterial fitness, this element drives a host specialization event - with improved fitness in Acanthamoeba but a reduced ability to replicate in other hosts and conditions. These observations add to a growing body of evidence that host range restriction can serve as an existential threat to L. pneumophila in the wild.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.