ArticleScientific reports2016
Molecular basis for the thermostability of Newcastle disease virus.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- Insights into the multifunctionality of viral glycoproteins F and HN in the lifecycle and pathogenesis of Newcastle disease virus: a systematic review.Veterinary research · 2025Pooled it
- Development and Characterization of a Thermostable Liquid Formulation of Live Newcastle Disease Vaccine.Veterinary sciences · 2026Article
- Global evolution dynamics of genotype VI NDVs and dissection of the biological properties of strains from the prevalent sub-genotypes.Journal of virology · 2026Article
- Stability of the two enveloped viruses NDV LaSota and YF-ZIKprM/E to support process development.PloS one · 2026Article
- Establishment of Vero cell-adapted thermostable candidate Newcastle Disease vaccine strain.Journal of advanced veterinary and animal research · 2025Article
- Residue 365 in Hemagglutinin-Neuraminidase Is a Key Thermostable Determinant of Genotype VI.2.1.1.2.2 Newcastle Disease Virus.Viruses · 2025Article
- Genetic and antigenic characteristics of genotype VII.1.1 Newcastle disease viruses currently circulating in Ethiopian chickens.Virology journal · 2025Article
- Recent advancements in the diverse roles of polymerase-associated proteins in the replication and pathogenesis of Newcastle disease virus.Veterinary research · 2025Review
- Oligoadenylate synthetase-like aggravated Newcastle disease virus-induced necroptosis in glioma cells.Frontiers in oncology · 2025Article
- Thermostability study of virulent Newcastle disease viruses isolated in Southern Angola.The Onderstepoort journal of veterinary research · 2024Article
- Quantitative regulation of the thermal stability of enveloped virus vaccines by surface charge engineering to prevent the self-aggregation of attachment glycoproteins.PLoS pathogens · 2022Article
- Root-preferential expression of Newcastle virus glycoproteins driven by NtREL1 promoter in tobacco hairy roots and evaluation of oral delivery in mice.Transgenic research · 2022Article
- Thermostable Vaccines in Veterinary Medicine: State of the Art and Opportunities to Be Seized.Vaccines · 2022Review
- Fusion Protein Cleavage Site Containing Three Basic Amino Acids Attenuates Newcastle Disease Virus in Chicken Embryos: Use as anFrontiers in microbiology · 2022Article
- Vaccine Quality Is a Key Factor to Determine Thermal Stability of Commercial Newcastle Disease (ND)Vaccines.Vaccines · 2021Article
- Article
- Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection.Pathogens (Basel, Switzerland) · 2020Article
- Generation and evaluation of a vaccine candidate of attenuated and heat-resistant genotype VIII Newcastle disease virus.Poultry science · 2020Article
- Residues 315 and 369 in HN Protein Contribute to the Thermostability of Newcastle Disease Virus.Frontiers in microbiology · 2020Article
- Genome Sequence of a Thermostable Avirulent Newcastle Disease Virus Isolated from Domestic Ducks in China.Microbiology resource announcements · 2019Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thermostable Newcastle disease virus (NDV) vaccines have been used widely to protect village chickens against Newcastle disease, due to their decreased dependence on cold chain for transport and storage. However, the genetic basis underlying the NDV thermostability is poorly understood. In this study, we generated chimeric viruses by exchanging viral genes between the thermostable TS09-C strain and thermolabile LaSota strain using reverse genetics technology. Evaluations of these chimeric NDVs demonstrated that the thermostability of NDV was dependent on the origin of HN protein. Chimeras bearing the HN protein derived from thermostable virus exhibited a thermostable phenotype, and vice versa. Both hemagglutinin and neuraminidase activities of viruses bearing the TS09-C HN protein were more thermostable than those containing LaSota HN protein. Furthermore, the newly developed thermostable virus rLS-T-HN, encoding the TS09-C HN protein in LaSota backbone, induced significantly higher antibody response than the TS09-C virus, and conferred complete protection against virulent NDV challenge. Taken together, the data suggest that the HN protein of NDV is a crucial determinant of thermostability, and the HN gene from a thermostable NDV could be engineered into a thermolabile NDV vaccine strain for developing novel thermostable NDV vaccine.
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