ArticleScientific reports2016
Cardiac Light-Sheet Fluorescent Microscopy for Multi-Scale and Rapid Imaging of Architecture and Function.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
55 citing papers in PubMed.
- Article
- Fetal Mouse Heart Clearing, Imaging, and 3D Reconstruction.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Emerging Light-Sheet Fluorescence Microscopy for Cardiac Vascular Imaging in Zebrafish.Current treatment options in cardiovascular medicine · 2026Review
- Advancements in Chitosan and Cellulose Nanoparticles for Stem Cell-Based Tissue Engineering.Stem cell reviews and reports · 2025Review
- Silicon nitride PIC beam formers for light sheet fluorescent microscopy.Biomedical optics express · 2025Article
- Refractive Index-Corrected Light-Sheet Microscopy for Macro-View Cardiovascular Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Volumetric imaging and computation to explore contractile function in zebrafish hearts.Cell reports methods · 2025Article
- An anatomically informed computational fluid dynamics modeling approach for quantifying hemodynamics in the developing heart.PloS one · 2025Article
- Cryo-X-Ray Phase Contrast Imaging Enables Combined 3D Structural Quantification and Nucleic Acid Analysis of Myocardial Biopsies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Frontiers in artificial intelligence-directed light-sheet microscopy for uncovering biological phenomena and multi-organ imaging.View (Beijing, China) · 2024Article
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
- Light-Sheet Imaging to Reveal Cardiac Structure in Rodent Hearts.Journal of visualized experiments : JoVE · 2024Article
- Assessing Cardiac Contractility From Single Molecules to Whole Hearts.JACC. Basic to translational science · 2024Review
- 4D Light-sheet imaging and interactive analysis of cardiac contractility in zebrafish larvae.APL bioengineering · 2023Article
- Quantitative 4D imaging of biomechanical regulation of ventricular growth and maturation.Current opinion in biomedical engineering · 2023Article
- Light sheet imaging and interactive analysis of the cardiac structure in neonatal mice.Journal of biophotonics · 2023Article
- Review
- Non-invasive photoacoustic computed tomography of rat heart anatomy and function.Light, science & applications · 2023Article
- Perspectives for Future Use of Cardiac Microtissues from Human Pluripotent Stem Cells.ACS biomaterials science & engineering · 2022Review
- Challenges and opportunities for the next generation of cardiovascular tissue engineering.Nature methods · 2022Review
Corrections and comments
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Authors and funding
16 authors.
Funding
Abstract
Light Sheet Fluorescence Microscopy (LSFM) enables multi-dimensional and multi-scale imaging via illuminating specimens with a separate thin sheet of laser. It allows rapid plane illumination for reduced photo-damage and superior axial resolution and contrast. We hereby demonstrate cardiac LSFM (c-LSFM) imaging to assess the functional architecture of zebrafish embryos with a retrospective cardiac synchronization algorithm for four-dimensional reconstruction (3-D space + time). By combining our approach with tissue clearing techniques, we reveal the entire cardiac structures and hypertrabeculation of adult zebrafish hearts in response to doxorubicin treatment. By integrating the resolution enhancement technique with c-LSFM to increase the resolving power under a large field-of-view, we demonstrate the use of low power objective to resolve the entire architecture of large-scale neonatal mouse hearts, revealing the helical orientation of individual myocardial fibers. Therefore, our c-LSFM imaging approach provides multi-scale visualization of architecture and function to drive cardiovascular research with translational implication in congenital heart diseases.
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Registered trials
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