ArticlePloS one2016
Intestinal Cell Tight Junctions Limit Invasion of Candida albicans through Active Penetration and Endocytosis in the Early Stages of the Interaction of the Fungus with the Intestinal Barrier.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 48 citations in OpenAlex.
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- The immune and microbial homeostasis determines theLife science alliance · 2024Article
- Reappraisal of intra-abdominal candidiasis: insights from peritoneal fluid analysis.Intensive care medicine experimental · 2023Article
- Recent Advances in Understanding the Human Fungal Pathogen Hypoxia Response in Disease Progression.Annual review of microbiology · 2023Review
- Relevance of biomarkers indicating gut damage and microbial translocation in people living with HIV.Frontiers in immunology · 2023Review
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- The Role of Host and Fungal Factors in the Commensal-to-Pathogen Transition ofCurrent clinical microbiology reports · 2023Review
- Trans-cellular tunnels induced by the fungal pathogen Candida albicans facilitate invasion through successive epithelial cells without host damage.Nature communications · 2022Article
- Assessing Als3 Peptide-Binding Cavity and Amyloid-Forming Region Contributions toFrontiers in cellular and infection microbiology · 2022Article
- Candidalysin triggers epithelial cellular stresses that induce necrotic death.Cellular microbiology · 2021Article
- In vitro infection models to study fungal-host interactions.FEMS microbiology reviews · 2021Review
- Tight Junctions as a Key for Pathogens Invasion in Intestinal Epithelial Cells.International journal of molecular sciences · 2021Review
- Antimicrobial Peptides Human Beta-Defensin-2 and -3 Protect the Gut DuringFrontiers in cellular and infection microbiology · 2021Article
- Mechanical force-induced morphology changes in a human fungal pathogen.BMC biology · 2020Article
- On Commensalism ofJournal of fungi (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
C. albicans is a commensal yeast of the mucous membranes in healthy humans that can also cause disseminated candidiasis, mainly originating from the digestive tract, in vulnerable patients. It is necessary to understand the cellular and molecular mechanisms of the interaction of C. albicans with enterocytes to better understand the basis of commensalism and pathogenicity of the yeast and to improve the management of disseminated candidiasis. In this study, we investigated the kinetics of tight junction (TJ) formation in parallel with the invasion of C. albicans into the Caco-2 intestinal cell line. Using invasiveness assays on Caco-2 cells displaying pharmacologically altered TJ (i.e. differentiated epithelial cells treated with EGTA or patulin), we were able to demonstrate that TJ protect enterocytes against invasion of C. albicans. Moreover, treatment with a pharmacological inhibitor of endocytosis decreased invasion of the fungus into Caco-2 cells displaying altered TJ, suggesting that facilitating access of the yeast to the basolateral side of intestinal cells promotes endocytosis of C. albicans in its hyphal form. These data were supported by SEM observations of differentiated Caco-2 cells displaying altered TJ, which highlighted membrane protrusions engulfing C. albicans hyphae. We furthermore demonstrated that Als3, a hypha-specific C. albicans invasin, facilitates internalization of the fungus by active penetration and induced endocytosis by differentiated Caco-2 cells displaying altered TJ. However, our observations failed to demonstrate binding of Als3 to E-cadherin as the trigger mechanism of endocytosis of C. albicans into differentiated Caco-2 cells displaying altered TJ.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.