Evidence map›Paper›PMID 26933040›Full record

ArticleStem cells translational medicine2016

Adipose-Derived Stem Cells Induce Angiogenesis via Microvesicle Transport of miRNA-31.

Ting Kang, Tia M Jones, Clayton Naddell, Methode Bacanamwo, John W Calvert, Winston E Thompson, Vincent C Bond, Y Eugene Chen, Dong Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells translational medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 144 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
144citing papers in PubMed, 5 pooled it
12.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

144 citing papers in PubMed, 5 syntheses or guidelines pooled it, 235 citations in OpenAlex.

  1. Exploring the landscape of stem cell extracellular vesicle research for angiogenesis: A bibliometric analysis from 2003 to 2023.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2024
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  6. Endogenous Engineering Reprograms Extracellular Vesicles for Enhanced Therapeutic Function.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
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84 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Ting KangDivision of Cardiology, The First Affiliated Hospital, Nanchang University, Nanchang, People's Republic of China Cardiovascular Research Institute, Morehouse School of Medicine, Atlanta, Georgia, USA.
Tia M JonesCardiovascular Research Institute, Morehouse School of Medicine, Atlanta, Georgia, USA.
Clayton NaddellCardiovascular Research Institute, Morehouse School of Medicine, Atlanta, Georgia, USA.
Methode BacanamwoCardiovascular Research Institute, Morehouse School of Medicine, Atlanta, Georgia, USA.
John W CalvertDivision of Cardiothoracic Surgery, Emory University School of Medicine, Atlanta, Georgia, USA.
Winston E ThompsonDepartment of Physiology, Morehouse School of Medicine, Atlanta, Georgia, USA.
Vincent C BondDepartment of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA.
Y Eugene ChenCardiovascular Center, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, Michigan, USA.
Dong LiuCardiovascular Research Institute, Morehouse School of Medicine, Atlanta, Georgia, USA Department of Physiology, Morehouse School of Medicine, Atlanta, Georgia, USA dliu@msm.edu.
Morehouse School of Medicine · USEmory University · USNanchang University · CNUniversity of Michigan–Ann Arbor · US

Funding

Morehouse School of Medicine Center of Excellence Research Endowment ProgramS21MD000101 · NIMHD · MOREHOUSE SCHOOL OF MEDICINE · PI MONTGOMERY-RICE, VALERIE C. · 2001 to 2015
$74.3M
TRANSCRIPTION FACTORS AND PROSTATE CANCERG12RR003034 · NCRR · MOREHOUSE SCHOOL OF MEDICINE · PI ZHAO, XUEYING · 1986 to 2011
$32.4M
Research Desogn and Biostatistics CoreU54MD007588 · NIMHD · MOREHOUSE SCHOOL OF MEDICINE · PI MAYBERRY, ROBERT M · 2012 to 2018
$24.8M
Proangiogenic microRNA in Microvesicles Released from Adipose-derived Stem CellsSC2GM099629 · NIGMS · MOREHOUSE SCHOOL OF MEDICINE · PI LIU, DONG · 2012 to 2014
$420k
NCRR NIH HHS G12 RR003034NCRR NIH HHS G12RR003034NIGMS NIH HHS SC2 GM099629NIGMS NIH HHS SC2GM099629NIMHD NIH HHS S21 MD000101NIMHD NIH HHS U54 MD007588
6 · The paper itself

Abstract

unlabelledCell secretion is an important mechanism for stem cell-based therapeutic angiogenesis, along with cell differentiation to vascular endothelial cells or smooth muscle cells. Cell-released microvesicles (MVs) have been recently implicated to play an essential role in intercellular communication. The purpose of this study was to explore the potential effects of stem cell-released MVs in proangiogenic therapy. We observed for the first time that MVs were released from adipose-derived stem cells (ASCs) and were able to increase the migration and tube formation of human umbilical vein endothelial cells (HUVECs). Endothelial differentiation medium (EDM) preconditioning of ASCs upregulated the release of MVs and enhanced the angiogenic effect of the released MVs in vitro. RNA analysis revealed that microRNA was enriched in ASC-released MVs and that the level of microRNA-31 (miR-31) in MVs was notably elevated upon EDM-preconditioning of MV-donor ASCs. Further studies exhibited that miR-31 in MVs contributed to the migration and tube formation of HUVECs, microvessel outgrowth of mouse aortic rings, and vascular formation of mouse Matrigel plugs. Moreover, factor-inhibiting HIF-1, an antiangiogenic gene, was identified as the target of miR-31 in HUVECs. Our findings provide the first evidence that MVs from ASCs, particularly from EDM-preconditioned ASCs, promote angiogenesis and the delivery of miR-31 may contribute the proangiogenic effect. SIGNIFICANCE: This study provides the evidence that microvesicles (MVs) from adipose-derived stem cells (ASCs), particularly from endothelial differentiation medium (EDM)-preconditioned ASCs, promote angiogenesis. An underlying mechanism of the proangiogenesis may be the delivery of microRNA-31 via MVs from ASCs to vascular endothelial cells in which factor-inhibiting HIF-1 is targeted and suppressed. The study findings reveal the role of MVs in mediating ASC-induced angiogenesis and suggest a potential MV-based angiogenic therapy for ischemic diseases.

Indexed as

Neovascularization, PhysiologicAdipose TissueAdult Stem CellsAnimalsCell-Derived MicroparticlesCell MovementCells, CulturedHumansHuman Umbilical Vein Endothelial CellsHypoxia-Inducible Factor 1, alpha SubunitMaleMiceMice, Inbred C57BLMice, NudeMicroRNAsMicrovesselsHif1a protein, mouseHypoxia-Inducible Factor 1, alpha SubunitMicroRNAsMirn31 microRNA, mouseAdipose stem cellAngiogenesisEndothelial cellMicrovesiclemiRNA

Identifiers

PMID26933040
PMCPMC4798737
OpenAlexW2288401268

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.