ArticleInternational journal of legal medicine2016
A SNP panel for identity and kinship testing using massive parallel sequencing.
Article in International journal of legal medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 74 citations in OpenAlex.
- Genetic Association Study Between Refractive Error-Related Genes and High Myopia in the Chinese Han Population.Journal of ophthalmology · 2026Article
- Characterization of the 172 SNPs Included in the ForenSeq™ DNA Signature Prep Kit in a Population from Northeast Italy.International journal of molecular sciences · 2025Article
- Complex kinship testing using the MGIEasy Pa-SNPs genotyping kit.International journal of legal medicine · 2025Article
- Predictive genetic panel for adult asthma using machine learning methods.The journal of allergy and clinical immunology. Global · 2024Article
- A novel approach of kinship determination based on the physical length of genetically shared regions of chromosomes.Genes & genomics · 2024Article
- A novel 193-plex MPS panel integrating STRs and SNPs highlights the application value of forensic genetics in individual identification and paternity testing.Human genetics · 2024Article
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- Evaluation of a custom QIAseq targeted DNA panel with 164 ancestry informative markers sequenced with the Illumina MiSeq.Scientific reports · 2021Article
- A novel forensic panel of 186-plex SNPs and 123-plex STR loci based on massively parallel sequencing.International journal of legal medicine · 2021Article
- A novel computational strategy to predict the value of the evidence in the SNP-based forensic mixtures.PloS one · 2021Article
- Population validation of reproductive gene mutation loci and association with the litter size in Nubian goat.Archives animal breeding · 2021Article
- Massive parallel sequencing in forensics: advantages, issues, technicalities, and prospects.International journal of legal medicine · 2020Review
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- The QIAGEN 140-locus single-nucleotide polymorphism (SNP) panel for forensic identification using massively parallel sequencing (MPS): an evaluation and a direct-to-PCR trial.International journal of legal medicine · 2019Article
- The Danish STR sequence database: duplicate typing of 363 Danes with the ForenSeq™ DNA Signature Prep Kit.International journal of legal medicine · 2019Article
- DNA identification of compromised samples with massive parallel sequencing.Forensic sciences research · 2019Article
- Massively parallel sequencing analysis of nondegraded and degraded DNA mixtures using the ForenSeq™ system in combination with EuroForMix software.International journal of legal medicine · 2019Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Within forensic genetics, there is still a need for supplementary DNA marker typing in order to increase the power to solve cases for both identity testing and complex kinship issues. One major disadvantage with current capillary electrophoresis (CE) methods is the limitation in DNA marker multiplex capability. By utilizing massive parallel sequencing (MPS) technology, this capability can, however, be increased. We have designed a customized GeneRead DNASeq SNP panel (Qiagen) of 140 previously published autosomal forensically relevant identity SNPs for analysis using MPS. One single amplification step was followed by library preparation using the GeneRead Library Prep workflow (Qiagen). The sequencing was performed on a MiSeq System (Illumina), and the bioinformatic analyses were done using the software Biomedical Genomics Workbench (CLC Bio, Qiagen). Forty-nine individuals from a Swedish population were genotyped in order to establish genotype frequencies and to evaluate the performance of the assay. The analyses showed to have a balanced coverage among the included loci, and the heterozygous balance showed to have less than 0.5 % outliers. Analyses of dilution series of the 2800M Control DNA gave reproducible results down to 0.2 ng DNA input. In addition, typing of FTA samples and bone samples was performed with promising results. Further studies and optimizations are, however, required for a more detailed evaluation of the performance of degraded and PCR-inhibited forensic samples. In summary, the assay offers a straightforward sample-to-genotype workflow and could be useful to gain information in forensic casework, for both identity testing and in order to solve complex kinship issues.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.