ArticleExtremophiles : life under extreme conditions2016
Conjugative plasmid transfer from Escherichia coli is a versatile approach for genetic transformation of thermophilic Bacillus and Geobacillus species.
Article in Extremophiles : life under extreme conditions, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 23 citations in OpenAlex.
- An Integrated System for Geobacillus Protoplast Formation, Transfection, Recombinant Genomes Rebooting, Efficient Propagation, and Genomic DNA Isolation of the Thermophilic Bacteriophage TP-84.MicrobiologyOpen · 2026Article
- The engineering toolbox of Parageobacillus thermoglucosidasius.Applied microbiology and biotechnology · 2025Review
- Efficient pathway-drivenApplied and environmental microbiology · 2024Article
- Development of a thermophilic host-vector system for the production of recombinant proteins at elevated temperatures.Applied microbiology and biotechnology · 2023Article
- Approaches to genetic tool development for rapid domestication of non-model microorganisms.Biotechnology for biofuels · 2021Review
- A plasmid vector that directs hyperproduction of recombinant proteins in the thermophiles Geobacillus species.Extremophiles : life under extreme conditions · 2020Article
- Transmating: conjugative transfer of a new broad host range expression vector to various Bacillus species using a single protocol.BMC microbiology · 2018Article
- Construction of a plasmid interspecific transfer system in Bacillus species with the counter-selectable marker mazF.Journal of industrial microbiology & biotechnology · 2018Article
- Genetic Tools and Techniques for Recombinant Expression in Thermophilic Bacillaceae.Microorganisms · 2018Review
- In vivo selection of sfGFP variants with improved and reliable functionality in industrially important thermophilic bacteria.Biotechnology for biofuels · 2018Article
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3 authors at 1 institution in 1 country.
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Abstract
We previously demonstrated efficient transformation of the thermophile Geobacillus kaustophilus HTA426 using conjugative plasmid transfer from Escherichia coli BR408. To evaluate the versatility of this approach to thermophile transformation, this study examined genetic transformation of various thermophilic Bacillus and Geobacillus spp. using conjugative plasmid transfer from E. coli strains. E. coli BR408 successfully transferred the E. coli-Geobacillus shuttle plasmid pUCG18T to 16 of 18 thermophiles with transformation efficiencies between 4.1 × 10(-7) and 3.8 × 10(-2)/recipient. Other E. coli strains that are different from E. coli BR408 in intracellular DNA methylation also generated transformants from 9 to 15 of the 18 thermophiles, including one that E. coli BR408 could not transform, although the transformation efficiencies of these strains were generally lower than those of E. coli BR408. The conjugation was performed by simple incubation of an E. coli donor and a thermophile recipient without optimization of experimental conditions. Moreover, thermophile transformants were distinguished from abundant E. coli donor only by high temperature incubation. These observations suggest that conjugative plasmid transfer, particularly using E. coli BR408, is a facile and versatile approach for plasmid introduction into thermophilic Bacillus and Geobacillus spp., and potentially a variety of other thermophiles.
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