ArticleParasites & vectors2016
Impact of Trypanosoma cruzi on antimicrobial peptide gene expression and activity in the fat body and midgut of Rhodnius prolixus.
Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 69 citations in OpenAlex.
- Expression of Proteins, Glycoproteins, and Transcripts in the Guts of Fasting, Fed, andPathogens (Basel, Switzerland) · 2023Pooled it
- Loss of vitellogenin receptor function results in yolk depletion, virome expansion, and reduced bacterial load within the oocytes of Rhodnius prolixus.BMC biology · 2026Article
- Food Deprivation inPathogens (Basel, Switzerland) · 2026Article
- Gut bacteria induce heterologous immune priming in Rhodnius prolixus encompassing both humoral and cellular immune responses.PLoS pathogens · 2025Article
- Regulation and Roles of Metacyclogenesis and Epimastigogenesis in the Life Cycle ofPathogens (Basel, Switzerland) · 2025Review
- Influence of the Origin, Feeding Status, andBiology · 2025Article
- Review
- Stable colonization of the kissing bug Rhodnius prolixus by Trypanosoma cruzi Y strain.PLoS neglected tropical diseases · 2025Article
- Azadirachtin disrupts ecdysone signaling and alters sand fly immunity.Parasites & vectors · 2024Article
- Interaction ofMicroorganisms · 2024Review
- Immune signaling pathways inFrontiers in physiology · 2024Article
- Evidence of a conserved mammalian immunosuppression mechanism inFrontiers in immunology · 2023Article
- Malpighian tubules ofFrontiers in insect science · 2023Review
- RNAi-mediated gene silencing ofFrontiers in physiology · 2023Article
- A fat body transcriptome analysis of the immune responses of Rhodnius prolixus to artificial infections with bacteria.Parasites & vectors · 2022Article
- The IMD and Toll canonical immune pathways of Triatoma pallidipennis are preferentially activated by Gram-negative and Gram-positive bacteria, respectively, but cross-activation also occurs.Parasites & vectors · 2022Article
- RNA as a feasible marker of Trypanosoma cruzi viability during the parasite interaction with the triatomine vector Rhodnius prolixus (Hemiptera, Triatominae).PLoS neglected tropical diseases · 2022Article
- Article
- Exposure to Trypanosoma parasites induces changes in the microbiome of the Chagas disease vector Rhodnius prolixus.Microbiome · 2022Article
- Inhibition of Zoonotic Pathogens Naturally Found in Pig Manure by Black Soldier Fly Larvae and Their Intestine Bacteria.Insects · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRhodnius prolixus is a major vector of Trypanosoma cruzi, the causative agent of Chagas disease in Latin America. In natural habitats, these insects are in contact with a variety of bacteria, fungi, virus and parasites that they acquire from both their environments and the blood of their hosts. Microorganism ingestion may trigger the synthesis of humoral immune factors, including antimicrobial peptides (AMPs). The objective of this study was to compare the expression levels of AMPs (defensins and prolixicin) in the different midgut compartments and the fat body of R. prolixus infected with different T. cruzi strains. The T. cruzi Dm 28c clone (TcI) successfully develops whereas Y strain (TcII) does not complete its life- cycle in R. prolixus. The relative AMP gene expressions were evaluated in the insect midgut and fat body infected on different days with the T. cruzi Dm 28c clone and the Y strain. The influence of the antibacterial activity on the intestinal microbiota was taken into account.
methodsThe presence of T. cruzi in the midgut of R. prolixus was analysed by optical microscope. The relative expression of the antimicrobial peptides encoding genes defensin (defA, defB, defC) and prolixicin (prol) was quantified by RT-qPCR. The antimicrobial activity of the AMPs against Staphylococcus aureus, Escherichia coli and Serratia marcescens were evaluated in vitro using turbidimetric tests with haemolymph, anterior and posterior midgut samples. Midgut bacteria were quantified using colony forming unit (CFU) assays and real time quantitative polymerase chain reaction (RT-qPCR).
resultsOur results showed that the infection of R. prolixus by the two different T. cruzi strains exhibited different temporal AMP induction profiles in the anterior and posterior midgut. Insects infected with T. cruzi Dm 28c exhibited an increase in defC and prol transcripts and a simultaneous reduction in the midgut cultivable bacteria population, Serratia marcescens and Rhodococcus rhodnii. In contrast, the T. cruzi Y strain neither induced AMP gene expression in the gut nor reduced the number of colony formation units in the anterior midgut. Beside the induction of a local immune response in the midgut after feeding R. prolixus with T. cruzi, a simultaneous systemic response was also detected in the fat body.
conclusionsR. prolixus AMP gene expressions and the cultivable midgut bacterial microbiota were modulated in distinct patterns, which depend on the T. cruzi genotype used for infection.
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