ArticleTransgenic research2016
Metabolic engineering of astaxanthin biosynthesis in maize endosperm and characterization of a prototype high oil hybrid.
Article in Transgenic research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 51 citations in OpenAlex.
- In Vivo Antioxidant Effects Evaluation of Genetically Modified Astaxanthin-Rich Maize.Foods (Basel, Switzerland) · 2026Article
- Metabolically engineered plant cell cultures as biofactories for the production of high-value carotenoids astaxanthin and canthaxanthin.Scientific reports · 2025Article
- Oilseed-based metabolic engineering of astaxanthin and related ketocarotenoids using a plant-derived pathway: Lab-to-field-to-application.Plant biotechnology journal · 2025Article
- Effect of Heterologous Expression of Key Enzymes Involved in Astaxanthin and Lipid Synthesis on Lipid and Carotenoid Production inMarine drugs · 2025Article
- Chlamydomonas reinhardtii, Volvox carteri and related green algae accumulate ketocarotenoids not in vegetative cells but in zygospores.The Plant journal : for cell and molecular biology · 2025Article
- Review
- Riboswitch-mediated inducible expression of an astaxanthin biosynthetic operon in plastids.Plant physiology · 2022Article
- Nucleotide variation in the phytoene synthase (ClPsy1) gene contributes to golden flesh in watermelon (Citrullus lanatus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Metabolomics of Chlorophylls and Carotenoids: Analytical Methods and Metabolome-Based Studies.Antioxidants (Basel, Switzerland) · 2021Review
- Metabolic engineering of astaxanthin-rich maize and its use in the production of biofortified eggs.Plant biotechnology journal · 2021Article
- The utility of metabolomics as a tool to inform maize biology.Plant communications · 2021Review
- Article
- Analyzing the action of evolutionarily conserved modules on HMW-GS 1Ax1 promoter activity.Plant molecular biology · 2020Article
- Heterologous expression ofPlant diversity · 2019Article
- The Diversity of Nutritional Metabolites: Origin, Dissection, and Application in Crop Breeding.Frontiers in plant science · 2019Review
- Engineering of tomato for the sustainable production of ketocarotenoids and its evaluation in aquaculture feed.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The Arabidopsis ORANGE (AtOR) gene promotes carotenoid accumulation in transgenic corn hybrids derived from parental lines with limited carotenoid pools.Plant cell reports · 2017Article
- Engineered maize as a source of astaxanthin: processing and application as fish feed.Transgenic research · 2016Article
- The distribution of carotenoids in hens fed on biofortified maize is influenced by feed composition, absorption, resource allocation and storage.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maize was genetically engineered for the biosynthesis of the high value carotenoid astaxanthin in the kernel endosperm. Introduction of a β-carotene hydroxylase and a β-carotene ketolase into a white maize genetic background extended the carotenoid pathway to astaxanthin. Simultaneously, phytoene synthase, the controlling enzyme of carotenogenesis, was over-expressed for enhanced carotenoid production and lycopene ε-cyclase was knocked-down to direct more precursors into the β-branch of the extended ketocarotenoid pathway which ends with astaxanthin. This astaxanthin-accumulating transgenic line was crossed into a high oil- maize genotype in order to increase the storage capacity for lipophilic astaxanthin. The high oil astaxanthin hybrid was compared to its astaxanthin producing parent. We report an in depth metabolomic and proteomic analysis which revealed major up- or down- regulation of genes involved in primary metabolism. Specifically, amino acid biosynthesis and the citric acid cycle which compete with the synthesis or utilization of pyruvate and glyceraldehyde 3-phosphate, the precursors for carotenogenesis, were down-regulated. Nevertheless, principal component analysis demonstrated that this compositional change is within the range of the two wild type parents used to generate the high oil producing astaxanthin hybrid.
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