ArticleInfection and immunity2016
Neisseria meningitidis Polynucleotide Phosphorylase Affects Aggregation, Adhesion, and Virulence.
Article in Infection and immunity, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 23 citations in OpenAlex.
- Molecular characterization of Neisseria meningitidis isolates from healthy individuals in Meigu County, Sichuan Province, 2021-2024.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Pilus-mediated co-aggregation with Lactobacillus crispatus increases meningococcal susceptibility to antimicrobial agents by interfering with microcolony formation.BMC microbiology · 2025Article
- Vertebrate and Invertebrate Animal and New In Vitro Models for StudyingPathogens (Basel, Switzerland) · 2023Review
- Developing New Tools to Fight Human Pathogens: A Journey through the Advances in RNA Technologies.Microorganisms · 2022Review
- Availability of polyamines affects virulence and survival of Neisseria meningitidis.Journal of microbiology (Seoul, Korea) · 2022Article
- Pseudomonas aeruginosa Polynucleotide Phosphorylase Controls Tolerance to Aminoglycoside Antibiotics by Regulating the MexXY Multidrug Efflux Pump.Antimicrobial agents and chemotherapy · 2021Article
- DNA Blocks the Lethal Effect of Human Beta-Defensin 2 AgainstFrontiers in microbiology · 2021Article
- Construction and immunization with double mutant ΔJournal of veterinary science · 2020Article
- RNase E Promotes Expression of Type III Secretion System Genes in Pseudomonas aeruginosa.Journal of bacteriology · 2019Article
- Pyrazinoic Acid Inhibits the Bifunctional Enzyme (Rv2783) inPathogens (Basel, Switzerland) · 2019Article
- Article
- Deletion of D-Lactate Dehydrogenase A inFrontiers in microbiology · 2019Article
- Polynucleotide phosphorylase: Not merely an RNase but a pivotal post-transcriptional regulator.PLoS genetics · 2018Review
- Novel Aspects of Polynucleotide Phosphorylase Function in Streptomyces.Antibiotics (Basel, Switzerland) · 2018Review
- Quantification ofBio-protocol · 2018Article
- Live-cell Imaging ofBio-protocol · 2018Article
- YeeJ is an inverse autotransporter from Escherichia coli that binds to peptidoglycan and promotes biofilm formation.Scientific reports · 2017Article
- A virulence-associated filamentous bacteriophage of Neisseria meningitidis increases host-cell colonisation.PLoS pathogens · 2017Article
- Host cell-derived lactate functions as an effector molecule in Neisseria meningitidis microcolony dispersal.PLoS pathogens · 2017Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neisseria meningitidis autoaggregation is an important step during attachment to human cells. Aggregation is mediated by type IV pili and can be modulated by accessory pilus proteins, such as PilX, and posttranslational modifications of the major pilus subunit PilE. The mechanisms underlying the regulation of aggregation remain poorly characterized. Polynucleotide phosphorylase (PNPase) is a 3'-5' exonuclease that is involved in RNA turnover and the regulation of small RNAs. In this study, we biochemically confirm that NMC0710 is the N. meningitidis PNPase, and we characterize its role in N. meningitidis pathogenesis. We show that deletion of the gene encoding PNPase leads to hyperaggregation and increased adhesion to epithelial cells. The aggregation induced was found to be dependent on pili and to be mediated by excessive pilus bundling. PNPase expression was induced following bacterial attachment to human cells. Deletion of PNPase led to global transcriptional changes and the differential regulation of 469 genes. We also demonstrate that PNPase is required for full virulence in an in vivo model of N. meningitidis infection. The present study shows that PNPase negatively affects aggregation, adhesion, and virulence in N. meningitidis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.