ArticleInfection and immunity2016
Cytotoxic and Inflammatory Responses Induced by Outer Membrane Vesicle-Associated Biologically Active Proteases from Vibrio cholerae.
Article in Infection and immunity, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 1 synthesis or guideline pooled it, 82 citations in OpenAlex.
- Bacterial extracellular vesicles - brain invaders? A systematic review.Frontiers in molecular neuroscience · 2023Pooled it
- Discovery and characterization of outer membrane vesicles produced by the bee gut microbiota.Biology letters · 2026Article
- The GlyGly-CTERM domain functions as an independent motif that targets proteins to rhombosortase inJournal of bacteriology · 2026Article
- New insights into outer membrane vesicles in Gram-negative bacteria from biogenesis to applications.Communications biology · 2026Review
- A peptide-induced regression of low-grade malignant breast tumor via activation of autophagy in breast cancer cells.Molecular medicine (Cambridge, Mass.) · 2026Article
- Sustained exposure to outer membrane vesicles from Escherichia coli develop mitochondrial oxidative stress and induce autophagy of macrophage.Archives of microbiology · 2026Article
- The GlyGly-CTERM domain functions as an independent motif that targets proteins to rhombosortase inbioRxiv : the preprint server for biology · 2026Article
- Outer membrane vesicles inMicrobial cell (Graz, Austria) · 2026Article
- Small molecule inhibitor targets OmpV to treat pandemic Vibrio cholerae infection.Nature communications · 2025Article
- Prevalence, Virulence and Antimicrobial Resistance ofFoods (Basel, Switzerland) · 2025Article
- Evaluating Outer Membrane Vesicle Isolation Techniques forAntibiotics (Basel, Switzerland) · 2025Article
- Hemagglutinin Protease HapA Associated With Vibrio cholerae Outer Membrane Vesicles (OMVs) Disrupts Tight and Adherens Junctions.Journal of extracellular vesicles · 2025Article
- Antigen 43 associated withMicrobiology spectrum · 2025Article
- Review on bacterial outer membrane vesicles: structure, vesicle formation, separation and biotechnological applications.Microbial cell factories · 2025Review
- Outer membrane vesicles in gram-negative bacteria and its correlation with pathogenesis.Frontiers in immunology · 2025Review
- Metallo-protease Peptidase M84 fromiScience · 2024Article
- Cargo exchange between human and bacterial extracellular vesicles in gestational tissues: a new paradigm in communication and immune development.Extracellular vesicles and circulating nucleic acids · 2024Review
- Microscopic messengers: microbiota-derived bacterial extracellular vesicles in inflammatory bowel disease.Frontiers in microbiology · 2024Review
- Clinical efficacy of washed microbiota transplantation on metabolic syndrome and metabolic profile of donor outer membrane vesicles.Frontiers in nutrition · 2024Article
- Bacterial Membrane Vesicles: Physiological Roles, Infection Immunology, and Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteases in Vibrio cholerae have been shown to play a role in its pathogenesis. V. cholerae secretes Zn-dependent hemagglutinin protease (HAP) and calcium-dependent trypsin-like serine protease (VesC) by using the type II secretion system (TIISS). Our present studies demonstrated that these proteases are also secreted in association with outer membrane vesicles (OMVs) and transported to human intestinal epithelial cells in an active form. OMV-associated HAP induces dose-dependent apoptosis in Int407 cells and an enterotoxic response in the mouse ileal loop (MIL) assay, whereas OMV-associated VesC showed a hemorrhagic fluid response in the MIL assay, necrosis in Int407 cells, and an increased interleukin-8 (IL-8) response in T84 cells, which were significantly reduced in OMVs from VesC mutant strain. Our results also showed that serine protease VesC plays a role in intestinal colonization of V. cholerae strains in adult mice. In conclusion, our study shows that V. cholerae OMVs secrete biologically active proteases which may play a role in cytotoxic and inflammatory responses.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.