ArticleProceedings of the National Academy of Sciences of the United States of America2016
Cyclic nucleotide-gated channel 18 is an essential Ca2+ channel in pollen tube tips for pollen tube guidance to ovules in Arabidopsis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
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90 citing papers in PubMed, 175 citations in OpenAlex.
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- CNGCs in Marchantia paleacea uncouple arbuscular mycorrhizal symbiosis and rhizoid development.The New phytologist · 2026Article
- Reactive Oxygen Species in Crop Plants: Production, Detoxification, Signaling, and Molecular Cross-Talk.Plants (Basel, Switzerland) · 2026Review
- A BRI1-CNGC12 phosphorylation module links hormone signaling to manganese homeostasis in plants.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Extracellular calcium modulates pollen tube growth and guidance inbioRxiv : the preprint server for biology · 2026Article
- Heat stress impact on rice reproductive processes: challenges and new approaches.Frontiers in genome editing · 2026Review
- The Gentiana rigescens genome and transcriptome for the investigation of flower repetitive blooming.BMC biology · 2025Article
- An intracellular CPK-ECA1 phosphoregulatory circuit couples calcium signatures to ABA homeostasis for plant osmosensivity.Science advances · 2025Article
- Genome-wide identification, characterization and expression analysis of CNGC Genes in moso bamboo (Phyllostachys edulis).BMC plant biology · 2025Article
- GmAKT1-mediated KPlant biotechnology journal · 2025Article
- Polarized subcellular activation of Rho proteins by specific ROPGEFs drives pollen germination in Arabidopsis thaliana.PLoS biology · 2025Article
- Cryo-EM structures of Arabidopsis CNGC1 and CNGC5 reveal molecular mechanisms underlying gating and calcium selectivity.Nature plants · 2025Article
- CPK1 activates CNGCs through phosphorylation for CaNature communications · 2025Article
- Genome-wide identification of cyclic nucleotide-gated channel gene family inFrontiers in plant science · 2025Article
- The crosstalk between nitrate signaling and other signaling molecules inFrontiers in plant science · 2025Review
- MID1-COMPLEMENTING ACTIVITY regulates cell proliferation and development via Ca2+ signaling in Marchantia polymorpha.Plant physiology · 2024Article
- The Role of Female and Male Genes in Regulating Pollen Tube Guidance in Flowering Plants.Genes · 2024Review
- Genome Analysis ofInternational journal of molecular sciences · 2024Article
- Differential phosphorylation of Ca2+-permeable channel CYCLIC NUCLEOTIDE-GATED CHANNEL20 modulates calcium-mediated freezing tolerance in Arabidopsis.The Plant cell · 2024Article
- Genome-Wide Identification and Expression Analysis of the Cyclic Nucleotide-Gated Channel Gene Family inInternational journal of molecular sciences · 2024Article
30 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In flowering plants, pollen tubes are guided into ovules by multiple attractants from female gametophytes to release paired sperm cells for double fertilization. It has been well-established that Ca(2+) gradients in the pollen tube tips are essential for pollen tube guidance and that plasma membrane Ca(2+) channels in pollen tube tips are core components that regulate Ca(2+) gradients by mediating and regulating external Ca(2+) influx. Therefore, Ca(2+) channels are the core components for pollen tube guidance. However, there is still no genetic evidence for the identification of the putative Ca(2+) channels essential for pollen tube guidance. Here, we report that the point mutations R491Q or R578K in cyclic nucleotide-gated channel 18 (CNGC18) resulted in abnormal Ca(2+) gradients and strong pollen tube guidance defects by impairing the activation of CNGC18 in Arabidopsis. The pollen tube guidance defects of cngc18-17 (R491Q) and of the transfer DNA (T-DNA) insertion mutant cngc18-1 (+/-) were completely rescued by CNGC18. Furthermore, domain-swapping experiments showed that CNGC18's transmembrane domains are indispensable for pollen tube guidance. Additionally, we found that, among eight Ca(2+) channels (including six CNGCs and two glutamate receptor-like channels), CNGC18 was the only one essential for pollen tube guidance. Thus, CNGC18 is the long-sought essential Ca(2+) channel for pollen tube guidance in Arabidopsis.
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