ArticleProceedings of the National Academy of Sciences of the United States of America2016
Two distinct cytokinesis pathways drive trypanosome cell division initiation from opposite cell ends.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 55 citations in OpenAlex.
- A kinesin-13 family kinesin in Trypanosoma brucei regulates cytokinesis and cytoskeleton morphogenesis by promoting microtubule bundling.PLoS pathogens · 2024Article
- Morphogenesis in Trypanosoma cruzi epimastigotes proceeds via a highly asymmetric cell division.PLoS neglected tropical diseases · 2023Article
- CRK2 controls cytoskeleton morphogenesis in Trypanosoma brucei by phosphorylating β-tubulin to regulate microtubule dynamics.PLoS pathogens · 2023Article
- Diversity in new flagellum tip attachment in bloodstream form African trypanosomes.Molecular microbiology · 2022Article
- Polo-like kinase and Aurora B kinase phosphorylate and cooperate with the CIF1-CIF2 complex to promote cytokinesis initiation inOpen biology · 2022Article
- KLIF-associated cytoskeletal proteins in Trypanosoma brucei regulate cytokinesis by promoting cleavage furrow positioning and ingression.The Journal of biological chemistry · 2022Article
- Article
- A novel lipase with dual localisation in Trypanosoma brucei.Scientific reports · 2022Article
- Revealing spatio-temporal dynamics with long-term trypanosomatid live-cell imaging.PLoS pathogens · 2022Article
- Drug Target Validation of the Protein KinaseMicrobiology spectrum · 2021Article
- Novel Cytoskeleton-Associated Proteins inMicroorganisms · 2021Article
- The kinetoplastid-specific phosphatase KPP1 attenuates PLK activity to facilitate flagellum inheritance inScience signaling · 2021Article
- Alternate histories of cytokinesis: lessons from the trypanosomatids.Molecular biology of the cell · 2020Review
- Role for the flagellum attachment zone in Leishmania anterior cell tip morphogenesis.PLoS pathogens · 2020Article
- Polo-like kinase in trypanosomes: an odd member out of the Polo family.Open biology · 2020Article
- FAZ27 cooperates with FLAM3 and ClpGM6 to maintain cell morphology inJournal of cell science · 2020Article
- Functional analyses of an axonemal inner-arm dynein complex in the bloodstream form of Trypanosoma brucei uncover its essential role in cytokinesis initiation.Molecular microbiology · 2019Article
- The trypanosome-specific proteins FPRC and CIF4 regulate cytokinesis initiation by recruiting CIF1 to the cytokinesis initiation site.The Journal of biological chemistry · 2019Article
- A kinetochore-based ATM/ATR-independent DNA damage checkpoint maintains genomic integrity in trypanosomes.Nucleic acids research · 2019Article
- BOH1 cooperates with Polo-like kinase to regulate flagellum inheritance and cytokinesis initiation inJournal of cell science · 2019Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Cytokinesis in Trypanosoma brucei, an early branching protozoan, occurs along its longitudinal axis uni-directionally from the anterior tip of the new flagellum attachment zone filament toward the cell's posterior end. However, the underlying mechanisms remain elusive. Here we report that cytokinesis in T. brucei is regulated by a concerted action of Polo-like kinase, Aurora B kinase, and a trypanosome-specific protein CIF1. Phosphorylation of CIF1 by Polo-like kinase targets it to the anterior tip of the new flagellum attachment zone filament, where it subsequently recruits Aurora B kinase to initiate cytokinesis. Consistent with its role, CIF1 depletion inhibits cytokinesis initiation from the anterior end of the cell, but, surprisingly, triggers cytokinesis initiation from the posterior end of the cell, suggesting the activation of an alternative cytokinesis from the opposite cell end. Our results reveal the mechanistic roles of CIF1 and Polo-like kinase in cytokinesis initiation and elucidate the mechanism underlying the recruitment of Aurora B kinase to the cytokinesis initiation site at late anaphase. These findings also delineate a signaling cascade controlling cytokinesis initiation from the anterior end of the cell and uncover a backup cytokinesis that is initiated from the posterior end of the cell when the typical anterior-to-posterior cytokinesis is compromised.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.