ArticleProceedings of the National Academy of Sciences of the United States of America2016
Novel genomic island modifies DNA with 7-deazaguanine derivatives.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
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90 citing papers in PubMed, 122 citations in OpenAlex.
- How bacterial immune systems sense phage infection.Nature reviews. Microbiology · 2026Review
- Structural and functional insights into QueC-family protein in QatABCD anti-phage system.Nature communications · 2026Article
- Antiviral defence is a conserved function of diverse bacterial DNA glycosylases.Nature microbiology · 2026Article
- Functional divergence and conservation in the QueC protein family (PF06508): from tRNA modification to anti-phage defense.The Biochemical journal · 2026Article
- Type III Druantia two-component antiphage defense depends on the DruH-DruE interaction for inhibiting viral DNA circularization and replication.Cell insight · 2026Article
- Structural and enzymatic insights into QatD, a dual-function TatD-like nuclease in the QatABCD anti-phage defense system.Nucleic acids research · 2026Article
- Complete genome sequence ofMicrobiology resource announcements · 2026Article
- Structural basis of QueC-family protein function in qatABCD anti-phage defense.Nature communications · 2026Article
- Functional Divergence and Conservation in the QueC Protein Family (PF06508): From tRNA Modification to Anti-Phage Defense.bioRxiv : the preprint server for biology · 2026Article
- It's not me, it's you: Anti-phage nuclease specificity inside a bacterium.PLoS pathogens · 2026Article
- Expanding the landscape of BREX diversity: uncovering multi-layered functional frameworks and identification of novel BREX-related defense systems.Nucleic acids research · 2026Article
- Phosphorothioate DNA modification by BREX type 4 systems in the human gut microbiome.Nature communications · 2026Article
- Biology of host-dependent restriction-modification in prokaryotes.EcoSal Plus · 2025Review
- Carboxymethylcytosine is a natural base modification and a handle for bacteriophage DNA hypermodification.Nature communications · 2025Article
- Deazaguanylation is a nucleobase-protein conjugation required for type IV CBASS immunity.Science (New York, N.Y.) · 2025Article
- YprA family helicases provide the missing link between diverse prokaryotic immune systems.bioRxiv : the preprint server for biology · 2025Article
- Structural basis of QueC-family protein function in qatABCD anti-phage defense.bioRxiv : the preprint server for biology · 2025Article
- Targeted genome mining with GATOR-GC maps the evolutionary landscape of biosynthetic diversity.Nucleic acids research · 2025Article
- Targeted genome mining with GATOR-GC maps the evolutionary landscape of biosynthetic diversity.bioRxiv : the preprint server for biology · 2025Article
- Nucleic acid recognition during prokaryotic immunity.Molecular cell · 2025Review
30 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors at 7 institutions in 4 countries.
Funding
Abstract
The discovery of ∼20-kb gene clusters containing a family of paralogs of tRNA guanosine transglycosylase genes, called tgtA5, alongside 7-cyano-7-deazaguanine (preQ0) synthesis and DNA metabolism genes, led to the hypothesis that 7-deazaguanine derivatives are inserted in DNA. This was established by detecting 2'-deoxy-preQ0 and 2'-deoxy-7-amido-7-deazaguanosine in enzymatic hydrolysates of DNA extracted from the pathogenic, Gram-negative bacteria Salmonella enterica serovar Montevideo. These modifications were absent in the closely related S. enterica serovar Typhimurium LT2 and from a mutant of S Montevideo, each lacking the gene cluster. This led us to rename the genes of the S. Montevideo cluster as dpdA-K for 7-deazapurine in DNA. Similar gene clusters were analyzed in ∼150 phylogenetically diverse bacteria, and the modifications were detected in DNA from other organisms containing these clusters, including Kineococcus radiotolerans, Comamonas testosteroni, and Sphingopyxis alaskensis Comparative genomic analysis shows that, in Enterobacteriaceae, the cluster is a genomic island integrated at the leuX locus, and the phylogenetic analysis of the TgtA5 family is consistent with widespread horizontal gene transfer. Comparison of transformation efficiencies of modified or unmodified plasmids into isogenic S. Montevideo strains containing or lacking the cluster strongly suggests a restriction-modification role for the cluster in Enterobacteriaceae. Another preQ0 derivative, 2'-deoxy-7-formamidino-7-deazaguanosine, was found in the Escherichia coli bacteriophage 9 g, as predicted from the presence of homologs of genes involved in the synthesis of the archaeosine tRNA modification. These results illustrate a deep and unexpected evolutionary connection between DNA and tRNA metabolism.
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