Evidence map›Paper›PMID 26929214›Full record

ArticleMolecular & cellular proteomics : MCP2016

Proteomic Analysis of the Mammalian Katanin Family of Microtubule-severing Enzymes Defines Katanin p80 subunit B-like 1 (KATNBL1) as a Regulator of Mammalian Katanin Microtubule-severing.

Keith Cheung, Silvia Senese, Jiaen Kuang, Ngoc Bui, Chayanid Ongpipattanakul, Ankur Gholkar, Whitaker Cohn, Joseph Capri, Julian P Whitelegge, Jorge Z Torres

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular & cellular proteomics : MCP, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
3.2field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 50 citations in OpenAlex.

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  10. Knockout ofInternational journal of molecular sciences · 2022
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  16. The Mammalian Family of Katanin Microtubule-Severing Enzymes.Frontiers in cell and developmental biology · 2021
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Keith CheungFrom the ‡Department of Chemistry and Biochemistry, University of California, Los Angeles, California, 90095;
Silvia SeneseFrom the ‡Department of Chemistry and Biochemistry, University of California, Los Angeles, California, 90095;
Jiaen KuangFrom the ‡Department of Chemistry and Biochemistry, University of California, Los Angeles, California, 90095;
Ngoc BuiFrom the ‡Department of Chemistry and Biochemistry, University of California, Los Angeles, California, 90095;
Chayanid OngpipattanakulFrom the ‡Department of Chemistry and Biochemistry, University of California, Los Angeles, California, 90095;
Ankur GholkarFrom the ‡Department of Chemistry and Biochemistry, University of California, Los Angeles, California, 90095;
Whitaker Cohn§Pasarow Mass Spectrometry Laboratory, The Jane and Terry Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California 90095;
Joseph Capri§Pasarow Mass Spectrometry Laboratory, The Jane and Terry Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California 90095;
Julian P Whitelegge§Pasarow Mass Spectrometry Laboratory, The Jane and Terry Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California 90095; ¶Molecular Biology Institute, University of California, Los Angeles, California, 90095; ‖Jonsson Comprehensive Cancer Center, University of California, Los Angeles, California, 90095.
Jorge Z TorresFrom the ‡Department of Chemistry and Biochemistry, University of California, Los Angeles, California, 90095; ¶Molecular Biology Institute, University of California, Los Angeles, California, 90095; ‖Jonsson Comprehensive Cancer Center, University of California, Los Angeles, California, 90095 torres@chem.ucla.edu.
University of California, Los Angeles · USUniversity of California System · US

Funding

Transgenic & Knock-out MouseP30DK063491 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI PETER J TONTONOZ · 2003 to 2026
$40.4M
UCLA MSD ProgramR25GM055052 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BARBER, PAUL HENRY, HASSON, TAMA W. · 1996 to 2021
$9.2M
NIDDK NIH HHS P30 DK063491NIGMS NIH HHS R25 GM055052
6 · The paper itself

Abstract

The Katanin family of microtubule-severing enzymes is critical for remodeling microtubule-based structures that influence cell division, motility, morphogenesis and signaling. Katanin is composed of a catalytic p60 subunit (A subunit, KATNA1) and a regulatory p80 subunit (B subunit, KATNB1). The mammalian genome also encodes two additional A-like subunits (KATNAL1 and KATNAL2) and one additional B-like subunit (KATNBL1) that have remained poorly characterized. To better understand the factors and mechanisms controlling mammalian microtubule-severing, we have taken a mass proteomic approach to define the protein interaction module for each mammalian Katanin subunit and to generate the mammalian Katanin family interaction network (Katan-ome). Further, we have analyzed the function of the KATNBL1 subunit and determined that it associates with KATNA1 and KATNAL1, it localizes to the spindle poles only during mitosis and it regulates Katanin A subunit microtubule-severing activity in vitro Interestingly, during interphase, KATNBL1 is sequestered in the nucleus through an N-terminal nuclear localization signal. Finally KATNB1 was able to compete the interaction of KATNBL1 with KATNA1 and KATNAL1. These data indicate that KATNBL1 functions as a regulator of Katanin A subunit microtubule-severing activity during mitosis and that it likely coordinates with KATNB1 to perform this function.

Indexed as

Adenosine TriphosphatasesCell NucleusHeLa CellsHumansKataninMass SpectrometryMeiosisMicrotubulesProtein Interaction MapsProteomicsAdenosine TriphosphatasesKataninKATNA1 protein, humanKATNAL1 protein, humanKATNB1 protein, humanKATNBL1 protein, human

Identifiers

PMID26929214
PMCPMC4858946
OpenAlexW2344953738

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.