ArticleChembiochem : a European journal of chemical biology2016
Development of Anionically Decorated Caged Neurotransmitters: In Vitro Comparison of 7-Nitroindolinyl- and 2-(p-Phenyl-o-nitrophenyl)propyl-Based Photochemical Probes.
Article in Chembiochem : a European journal of chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed.
- Blue and Green Light Responsive Caged Glutamate.Journal of photochemistry and photobiology. A, Chemistry · 2024Article
- Reverse Engineering Caged Compounds: Design Principles for their Application in Biology.Angewandte Chemie (International ed. in English) · 2023Review
- Cloaked caged glutamate eliminates off-target GABA-A receptor antagonism and opens a new door in neuroscience.Lab animal · 2020Article
- Optofluidic control of rodent learning using cloaked caged glutamate.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Chromatically independent, two-color uncaging of glutamate and GABA with one- or two-photon excitation.Methods in enzymology · 2019Article
- Thermodynamically Stable, Photoreversible Pharmacology in Neurons with One- and Two-Photon Excitation.Angewandte Chemie (International ed. in English) · 2018Article
- Comparative one- and two-photon uncaging of MNI-glutamate and MNI-kainate on hippocampal CA1 neurons.Journal of neuroscience methods · 2018Article
- Two-Photon Uncaging of Glutamate.Frontiers in synaptic neuroscience · 2018Review
- Two-color, one-photon uncaging of glutamate and GABA.PloS one · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Neurotransmitter uncaging, especially that of glutamate, has been used to study synaptic function for over 30 years. One limitation of caged glutamate probes is the blockade of γ-aminobutyric acid (GABA)-A receptor function. This problem comes to the fore when the probes are applied at the high concentrations required for effective two-photon photolysis. To mitigate such problems one could improve the photochemical properties of caging chromophores and/or remove receptor blockade. We show that addition of a dicarboxylate unit to the widely used 4-methoxy-7-nitroindolinyl-Glu (MNI-Glu) system reduced the off-target effects by about 50-70 %. When the same strategy was applied to an electron-rich 2-(p-Phenyl-o-nitrophenyl)propyl (PNPP) caging group, the pharmacological improvements were not as significant as in the MNI case. Finally, we used very extensive biological testing of the PNPP-caged Glu (more than 250 uncaging currents at single dendritic spines) to show that nitro-biphenyl caging chromophores have two-photon uncaging efficacies similar to that of MNI-Glu.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.