ArticleContrast media & molecular imaging2016
Evaluating the effectiveness of transferrin receptor-1 (TfR1) as a magnetic resonance reporter gene.
Article in Contrast media & molecular imaging, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Article
- Advances in iron-storage protein-based MRI reporter genes: from molecular mechanisms to translational imaging applications.Frontiers in bioengineering and biotechnology · 2026Review
- Exploring the potential of water channels for developing genetically encoded reporters and biosensors for diffusion-weighted MRI.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2024Article
- Engineering water exchange is a safe and effective method for magnetic resonance imaging in diverse cell types.Journal of biological engineering · 2024Article
- Exploring the potential of water channels for developing MRI reporters and sensors without the need for exogenous contrast agents.bioRxiv : the preprint server for biology · 2024Article
- Engineering water exchange is a safe and effective method for magnetic resonance imaging in diverse cell types.bioRxiv : the preprint server for biology · 2023Article
- Optical and MRI Multimodal Tracing of Stem Cells In Vivo.Molecular imaging · 2023Review
- A brief review of reporter gene imaging in oncolytic virotherapy and gene therapy.Molecular therapy oncolytics · 2021Review
- Article
- Beyond the Green Fluorescent Protein: Biomolecular Reporters for Anaerobic and Deep-Tissue Imaging.Bioconjugate chemistry · 2020Review
- Dual-Effect of Magnetic Resonance Imaging Reporter Gene in Diagnosis and Treatment of Hepatocellular Carcinoma.International journal of nanomedicine · 2020Article
- Optical fluorescence imaging with shortwave infrared light emitter nanomaterials for in vivo cell tracking in regenerative medicine.Journal of cellular and molecular medicine · 2019Review
- MR molecular imaging of HCC employing a regulated ferritin gene carried by a modified polycation vector.International journal of nanomedicine · 2019Article
- Characterization of Magneto-Endosymbionts as MRI Cell Labeling and Tracking Agents.Molecular imaging and biology · 2018Article
- Article
- Preclinical imaging methods for assessing the safety and efficacy of regenerative medicine therapies.NPJ Regenerative medicine · 2017Review
- Imaging technologies for monitoring the safety, efficacy and mechanisms of action of cell-based regenerative medicine therapies in models of kidney disease.European journal of pharmacology · 2016Review
- MRI Reporter Genes for Noninvasive Molecular Imaging.Molecules (Basel, Switzerland) · 2016Review
- Evaluating the effectiveness of transferrin receptor-1 (TfR1) as a magnetic resonance reporter gene.Contrast media & molecular imaging · 2016Article
- MS-1 magA: Revisiting Its Efficacy as a Reporter Gene for MRI.Molecular imaging · 2016Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Magnetic resonance (MR) reporter genes have the potential for tracking the biodistribution and fate of cells in vivo, thus allowing the safety, efficacy and mechanisms of action of cell-based therapies to be comprehensively assessed. In this study, we evaluate the effectiveness of the iron importer transferrin receptor-1 (TfR1) as an MR reporter gene in the model cell line CHO-K1. Overexpression of the TfR1 transgene led to a reduction in the levels of endogenous TfR1 mRNA, but to a 60-fold increase in total TfR1 protein levels. Although the mRNA levels of ferritin heavy chain-1 (Fth1) did not change, Fth1 protein levels increased 13-fold. The concentration of intracellular iron increased significantly, even when cells were cultured in medium that was not supplemented with iron and the amount of iron in the extracellular environment was thus at physiological levels. However, we found that, by supplementing the cell culture medium with ferric citrate, a comparable degree of iron uptake and MR contrast could be achieved in control cells that did not express the TfR1 transgene. Sufficient MR contrast to enable the cells to be detected in vivo following their administration into the midbrain of chick embryos was obtained irrespective of the reporter gene. We conclude that TfR1 is not an effective reporter and that, to track the biodistribution of cells with MR imaging in the short term, it is sufficient to simply culture cells in the presence of ferric citrate. Copyright © 2016 The Authors Contrast Media & Molecular Imaging Published by John Wiley & Sons Ltd.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.