ArticleNature chemical biology2016
Direct observation of intermediate states during the stepping motion of kinesin-1.
Article in Nature chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 158 citations in OpenAlex.
- Anisotropic unbinding and location-dependent hovering of a kinesin motor head over microtubule.Biophysical journal · 2026Article
- Neck region-microtubule interactions direct counterclockwise stepping of kinesin-1.Biophysical journal · 2026Article
- An Overview of Contrasting Experimental Results on Dynamics of Kinesin-1 Molecular Motors: Insight into the Underlying Mechanism.Biomolecules · 2025Review
- Tension-induced suppression of allosteric conformational changes coordinates kinesin-1 stepping.The Journal of cell biology · 2025Article
- Sustainable Synthesis, Characterization, Cellular Effects of Gold Nanoparticles and Their Applications as Therapeutics in Cancer Therapy.ChemPlusChem · 2025Review
- Factors Determining Kinesin Motors in a Predominant One-Head-Bound or Two-Heads-Bound State During Its Stepping Cycle.Biomolecules · 2025Article
- Kinetic regulation of kinesin's two motor domains coordinates its stepping along microtubules.eLife · 2025Article
- MINFLUX achieves molecular resolution with minimal photons.Nature photonics · 2025Article
- Impact of physiological ionic strength and crowding on kinesin-1 motility.Cell structure and function · 2025Article
- Multiple kinesins speed up cargo transport in crowded environments by sharing load.Communications biology · 2025Article
- Mechanism and regulation of kinesin motors.Nature reviews. Molecular cell biology · 2025Review
- Rational engineering of DNA-nanoparticle motor with high speed and processivity comparable to motor proteins.Nature communications · 2025Article
- Review
- Biased movement of monomeric kinesin-3 KLP-6 explained by a symmetric Brownian ratchet model.Biophysical journal · 2025Article
- Tracking Single Kinesin in Live Cells Using MINFLUX.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Visualizing Single V-ATPase Rotation Using Janus Nanoparticles.Nano letters · 2024Article
- Nonthermal fluctuations accelerate biomolecular motors.Biophysical reviews · 2024Review
- Visualizing Single V-ATPase Rotation Using Janus Nanoparticles.bioRxiv : the preprint server for biology · 2024Article
- Uncovering kinesin dynamics in neurites with MINFLUX.Communications biology · 2024Article
- A Model for Chemomechanical Coupling of Kinesin-3 Motor.Cellular and molecular bioengineering · 2024Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dimeric motor protein kinesin-1 walks along microtubules by alternatingly hydrolyzing ATP and moving two motor domains ('heads'). Nanometer-precision single-molecule studies demonstrated that kinesin takes regular 8-nm steps upon hydrolysis of each ATP; however, the intermediate states between steps have not been directly visualized. Here, we employed high-temporal resolution dark-field microscopy to directly visualize the binding and unbinding of kinesin heads to or from microtubules during processive movement. Our observations revealed that upon unbinding from microtubules, the labeled heads were displaced rightward and underwent tethered diffusive movement. Structural and kinetic analyses of wild-type and mutant kinesins with altered neck linker lengths provided evidence that rebinding of the unbound head to the rear-binding site is prohibited by a tension increase in the neck linker and that ATP hydrolysis by the leading head is suppressed when both heads are bound to the microtubule, thereby explaining how the two heads coordinate to move in a hand-over-hand manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.