ArticleJournal of chromatography. A2016
Comprehensive untargeted lipidomic analysis using core-shell C30 particle column and high field orbitrap mass spectrometer.
Article in Journal of chromatography. A, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed.
- Intrahepatic Fat and Postprandial Glycemia Increase After Consumption of a Diet Enriched in Saturated Fat Compared With Free Sugars.Diabetes care · 2020Trial
- Implementation of Nano Flow Chromatography Coupled to Mass Spectrometry as a Reliable and Sensitive Discovery Lipidomics Platform.Rapid communications in mass spectrometry : RCM · 2026Article
- Making Ozonolysis Mass Spectrometry More Accessible: A Simple LC-OzESI-MS Workflow for Carbon-Carbon Double Bond Position Elucidation in Lipids and Natural Products.Journal of the American Society for Mass Spectrometry · 2026Article
- TRIS-Egg Yolk Extender Attenuates Seminolipid and Sphingomyelin Loss in Chilled Canine Semen: An LC-HRMS Lipidomic and CASA Study.Animals : an open access journal from MDPI · 2026Article
- Article
- Lipidomic Signatures in Pediatric Metabolic Disorders.Metabolites · 2025Review
- LncRNA CRCMSL interferes in phospholipid unsaturation to suppress colorectal cancer progression via reducing membrane fluidity.Journal of advanced research · 2025Article
- Serum triglyceride and apolipoprotein A1 as dynamic biomarkers in extranodal NK/T-cell lymphoma (ENKTL): A multicenter retrospective and prospective validation study.Annals of hematology · 2025Article
- Effects of Lysolecithin on Growth Performance, Antioxidant Capacity, and Lipid Metabolism ofAntioxidants (Basel, Switzerland) · 2025Article
- Urinary 3-methylhistidine as a potential biomarker for sepsis-associated acute kidney injury: multidimensional metabolomics analysis in mice and human.Annals of intensive care · 2025Article
- Lipidomic Profiling Reveals HSD17B13 Deficiency-Associated Dysregulated Hepatic Phospholipid Metabolism in Aged Mice.Metabolites · 2025Article
- Influence of fermented feed on lipid composition and volatile flavor profile of duck eggs: Insights from multi-omics analysis.Food chemistry: X · 2025Article
- Interleukin-12 signaling drives Alzheimer's disease pathology through disrupting neuronal and oligodendrocyte homeostasis.Nature aging · 2025Article
- OzNOxESI: A Novel Mass Spectrometry Ion Chemistry for Elucidating Lipid Double-Bond Regioisomerism in Complex Mixtures.Analytical chemistry · 2025Article
- Zhuriheng pills improve adipose tissue dysfunction and inflammation by modulating PPARγ to stabilize atherosclerotic plaques.Frontiers in pharmacology · 2025Article
- Tandem Mass Spectrometry in Untargeted Lipidomics: A Case Study of Peripheral Blood Mononuclear Cells.International journal of molecular sciences · 2024Article
- Triptolide induces hepatotoxicity by promoting ferroptosis through Nrf2 degradation.Cell biology and toxicology · 2024Article
- Comparative Lipidomics Analysis Provides New Insights into the Metabolic Basis of Color Formation in Green Cotton Fiber.Plants (Basel, Switzerland) · 2024Article
- Digitally Enabled Generic Analytical Framework Accelerating the Pace of Liquid Chromatography Method Development for Vaccine Adjuvant Formulations.ACS pharmacology & translational science · 2024Article
- Quantifying acyl-chain diversity in isobaric compound lipids containing monomethyl branched-chain fatty acids.bioRxiv : the preprint server for biology · 2024Article
19 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors.
Funding
Abstract
The goal of untargeted lipidomics is to have high throughput, yet comprehensive and unambiguous identification and quantification of lipids. Novel stationary phases in LC separation and new mass spectrometric instruments capable of high mass resolving power and faster scanning rate are essential to achieving this goal. In this work, 4 reversed phase LC columns coupled with a high field quadrupole orbitrap mass spectrometer (Q Exactive HF) were thoroughly compared using complex lipid standard mixture and rat plasma and liver samples. A good separation of all lipids was achieved in 24min of gradient. The columns compared include C30 and C18 functionalization on either core-shell or totally porous silica particles, with size ranging from 1.7 to 2.6μm. Accucore C30 column showed the narrowest peaks and highest theoretical plate number, and excellent peak capacity and retention time reproducibility (<1% standard deviation). As a result, it resulted in 430 lipid species identified from rat plasma and rat liver samples with highest confidence. The high resolution offered by the up-front RPLC allowed discrimination of cis/trans isomeric lipid species, and the high field orbitrap mass spectrometer afforded the clear distinction of isobaric lipid species in full scan MS and the unambiguous assignment of sn-positional isomers for lysophospholipids in MS/MS. Taken together, the high efficiency LC separation and high mass resolving MS analysis are very promising tools for untargeted lipidomics analysis.
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