ArticleNature cell biology2016
Blood flow drives lumen formation by inverse membrane blebbing during angiogenesis in vivo.
Article in Nature cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
103 citing papers in PubMed, 203 citations in OpenAlex.
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- MCC links Wnt/PCP signaling to endothelial polarity and vascular remodeling.Angiogenesis · 2026Article
- Hydraulic fracturing-induced delamination and extravasation extends medial damage beyond the false lumen in aortic dissection.bioRxiv : the preprint server for biology · 2026Article
- Ex vivo imaging reveals neutrophil behaviors in the adult zebrafish heart.Journal of cell science · 2026Article
- Automated analysis of zebrafish vascular networks using the VISTA-Z pipeline.Scientific reports · 2026Article
- Complex relationship among vessel diameter, shear stress and blood pressure controlling vessel pruning during angiogenesis.PLoS computational biology · 2026Article
- Highly divergent apicomplexan cytoskeletons provide additional models for actin biology.The FEBS journal · 2025Review
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- A developmental atlas of zebrafish gills links early vascular patterning to adult architecture.Development (Cambridge, England) · 2025Article
- Biomechanical control of vascular morphogenesis by the surrounding stiffness.Nature communications · 2025Article
- Progress in recapitulating morphogenesis of blood microvascular structures for microphysiological systems development.Biochemical Society transactions · 2025Article
- Physics of notochord tube expansion in ascidians.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Reversal of cerebrovascular anomalies in a zebrafish model of vein of Galen aneurysm.Nature cardiovascular research · 2025Article
- Chemokine induces phase transition from non-directional to directional migration during angiogenesis.Cell structure and function · 2025Article
- Article
- Oscillatory contractile forces refine endothelial cell-cell interactions for continuous lumen formation governed by Heg1/Ccm1.Angiogenesis · 2024Article
- Inverse bleb membrane protrusions pump fluid within the early mouse embryo.Nature cell biology · 2024Article
- Inverse blebs operate as hydraulic pumps during mouse blastocyst formation.Nature cell biology · 2024Article
- The life cycle of a capillary: Mechanisms of angiogenesis and rarefaction in microvascular physiology and pathologies.Vascular pharmacology · 2024Review
43 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 3 institutions in 4 countries.
Funding
Abstract
How vascular tubes build, maintain and adapt continuously perfused lumens to meet local metabolic needs remains poorly understood. Recent studies showed that blood flow itself plays a critical role in the remodelling of vascular networks, and suggested it is also required for the lumenization of new vascular connections. However, it is still unknown how haemodynamic forces contribute to the formation of new vascular lumens during blood vessel morphogenesis. Here we report that blood flow drives lumen expansion during sprouting angiogenesis in vivo by inducing spherical deformations of the apical membrane of endothelial cells, in a process that we have termed inverse blebbing. We show that endothelial cells react to these membrane intrusions by local and transient recruitment and contraction of actomyosin, and that this mechanism is required for single, unidirectional lumen expansion in angiogenic sprouts. Our work identifies inverse membrane blebbing as a cellular response to high external pressure. We show that in the case of blood vessels such membrane dynamics can drive local cell shape changes required for global tissue morphogenesis, shedding light on a pressure-driven mechanism of lumen formation in vertebrates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.