Evidence map›Paper›PMID 26928722›Full record

ArticleBMC research notes2016

Unbiased profiling of volatile organic compounds in the headspace of Allium plants using an in-tube extraction device.

Miyako Kusano, Makoto Kobayashi, Yumiko Iizuka, Atsushi Fukushima, Kazuki Saito

Abstract read
In one paragraph

Article in BMC research notes, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

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  13. Profiling of volatile flavor compounds inFood science & nutrition · 2018
    Article
  14. Rapid Screening of Volatile Organic Compounds fromInternational journal of analytical chemistry · 2018
    Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Miyako KusanoGraduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai, Tsukuba, Ibaraki, Japan. kusano.miyako.fp@u.tsukuba.ac.jp.
Makoto KobayashiRIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan. kobamako@riken.jp.
Yumiko IizukaRIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan. yiizuka5@gmail.com.
Atsushi FukushimaRIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan. atsushi.fukushima@riken.jp.
Kazuki SaitoRIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan. kazuki.saito@riken.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPlants produce and emit important volatile organic compounds (VOCs), which have an essential role in biotic and abiotic stress responses and in plant-plant and plant-insect interactions. In order to study the bouquets from plants qualitatively and quantitatively, a comprehensive, analytical method yielding reproducible results is required.

resultsWe applied in-tube extraction (ITEX) and solid-phase microextraction (SPME) for studying the emissions of Allium plants. The collected HS samples were analyzed by gas chromatography-time-of-flight-mass spectrometry (GC-TOF-MS), and the results were subjected to multivariate analysis. In case of ITEX-method Allium cultivars released more than 300 VOCs, out of which we provisionally identified 50 volatiles. We also used the VOC profiles of Allium samples to discriminate among groups of A. fistulosum, A. chinense (rakkyo), and A. tuberosum (Oriental garlic). As we found 12 metabolite peaks including dipropyl disulphide with significant changes in A. chinense and A. tuberosum when compared to the control cultivar, these metabolite peaks can be used for chemotaxonomic classification of A. chinense, tuberosum, and A. fistulosum.

conclusionsCompared to SPME-method our ITEX-based VOC profiling technique contributes to automatic and reproducible analyses. Hence, it can be applied to high-throughput analyses such as metabolite profiling.

Indexed as

AlliumGas Chromatography-Mass SpectrometryMetabolomePlant Components, AerialPlant ExtractsSolid Phase MicroextractionSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationVolatile Organic CompoundsPlant ExtractsVolatile Organic Compounds

Identifiers

PMID26928722
PMCPMC4772445

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.