ArticlePLoS neglected tropical diseases2016
High Resolution Melting Analysis Targeting hsp70 as a Fast and Efficient Method for the Discrimination of Leishmania Species.
Article in PLoS neglected tropical diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 72 citations in OpenAlex.
- High resolution melting real-time PCR for genotyping of Giardia lamblia assemblages A and B regardless of parasite load.Gut pathogens · 2025Article
- Ecological Perspectives on Leishmaniasis Parasitism Patterns: Evidence of Possible Alternative Vectors forPathogens (Basel, Switzerland) · 2025Article
- First Report of Rhodnius stali Lent, Jurberg, and Galvão 1993 (Hemiptera: Reduviidae: Triatominae) Naturally Infected by Trypanosoma cruzi in Concepción Department, Paraguay.The American journal of tropical medicine and hygiene · 2025Article
- Diversity of Anaplasmataceae Transmitted by Ticks (Ixodidae) and the First Molecular Evidence ofMicroorganisms · 2024Article
- Assessment of High-Resolution Melting Curve Analysis forPathogens (Basel, Switzerland) · 2024Article
- Detection of Internal Transcribed Spacer 1 and hsp70 Genetic Markers Using Restriction Fragment Length Polymorphisms and Sequencing in Identification of Leishmania Species Causing Tegumentary Leishmaniasis in Brazil.The American journal of tropical medicine and hygiene · 2024Article
- Characterisation of an area of coexistent visceral and cutaneous leishmaniasis transmission in the State of Piauí, Brazil.Memorias do Instituto Oswaldo Cruz · 2024Article
- HRM Accuracy and Limitations as a Species Typing Tool forInternational journal of molecular sciences · 2023Article
- Parasite Detection in Visceral Leishmaniasis Samples by Dye-Based qPCR Using New Gene Targets ofTropical medicine and infectious disease · 2023Article
- Detection ofAnimals : an open access journal from MDPI · 2023Article
- Evaluation of Molecular Methods to Identify Chagas Disease and Leishmaniasis in Blood Donation Candidates in Two Brazilian Centers.Pathogens (Basel, Switzerland) · 2023Article
- Evaluation of the Diagnostic Sensitivity of the VIASURETransboundary and emerging diseases · 2023Article
- Accurate Identification ofJournal of arthropod-borne diseases · 2022Article
- High-resolution melting analysis identifies reservoir hosts of zoonotic Leishmania parasites in Tunisia.Parasites & vectors · 2022Article
- Preliminary observations of the urbanization and domiciliation of the American cutaneous leishmaniasis in Rio Branco, Acre, Western Amazon.Revista da Sociedade Brasileira de Medicina Tropical · 2022Article
- Multiplex high-resolution melting assay for simultaneous detection of five key bacterial pathogens in urinary tract infections: A pilot study.Frontiers in microbiology · 2022Article
- Visceral leishmaniasis caused by Leishmania (Leishmania) amazonensis associated with Hodgkin's lymphoma.Revista do Instituto de Medicina Tropical de Sao Paulo · 2022Article
- Sensitivity comparison for the Leishmania spp. detection in different canine tissues using PCR-HRM.Revista da Sociedade Brasileira de Medicina Tropical · 2022Article
- Two Leishmania species separation targeting the ITS-rDNA and Cyt b genes by developing and evaluating HRM- qPCR.Revista da Sociedade Brasileira de Medicina Tropical · 2022Article
- High resolution melting assay in discrimination of the main etiologic agents of leishmaniasis in Iran.Iranian journal of microbiology · 2021Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundProtozoan parasites of the genus Leishmania cause a large spectrum of clinical manifestations known as Leishmaniases. These diseases are increasingly important public health problems in many countries both within and outside endemic regions. Thus, an accurate differential diagnosis is extremely relevant for understanding epidemiological profiles and for the administration of the best therapeutic protocol. METHODS/PRINCIPAL
findingsExploring the High Resolution Melting (HRM) dissociation profiles of two amplicons using real time polymerase chain reaction (real-time PCR) targeting heat-shock protein 70 coding gene (hsp70) revealed differences that allowed the discrimination of genomic DNA samples of eight Leishmania species found in the Americas, including Leishmania (Leishmania) infantum chagasi, L. (L.) amazonensis, L. (L.) mexicana, L. (Viannia) lainsoni, L. (V.) braziliensis, L. (V.) guyanensis, L. (V.) naiffi and L. (V.) shawi, and three species found in Eurasia and Africa, including L. (L.) tropica, L. (L.) donovani and L. (L.) major. In addition, we tested DNA samples obtained from standard promastigote culture, naturally infected phlebotomines, experimentally infected mice and clinical human samples to validate the proposed protocol. CONCLUSIONS/SIGNIFICANCE: HRM analysis of hsp70 amplicons is a fast and robust strategy that allowed for the detection and discrimination of all Leishmania species responsible for the Leishmaniases in Brazil and Eurasia/Africa with high sensitivity and accuracy. This method could detect less than one parasite per reaction, even in the presence of host DNA.
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