ArticleBioorganic & medicinal chemistry letters2016
Synthesis and evaluation of an alkyne-modified ATP analog for enzymatic incorporation into RNA.
Article in Bioorganic & medicinal chemistry letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Article
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- Functionalised Cofactor Mimics for Interactome Discovery and Beyond.Angewandte Chemie (International ed. in English) · 2022Review
- Exploiting Endogenous Enzymes for Cancer-Cell Selective Metabolic Labeling of RNA in Vivo.Journal of the American Chemical Society · 2022Article
- Total RNA Synthesis and its Covalent Labeling Innovation.Topics in current chemistry (Cham) · 2022Review
- Triazole-Modified Nucleic Acids for the Application in Bioorganic and Medicinal Chemistry.Biomedicines · 2021Review
- A Bump-Hole Strategy for Increased Stringency of Cell-Specific Metabolic Labeling of RNA.ACS chemical biology · 2020Article
- Modified nucleoside triphosphates in bacterial research forRSC chemical biology · 2020Article
- Bioorthogonal chemistry-based RNA labeling technologies: evolution and current state.Chemical communications (Cambridge, England) · 2020Article
- An optimized chemical-genetic method for cell-specific metabolic labeling of RNA.Nature methods · 2020Article
- Expanding the Scope of RNA Metabolic Labeling with Vinyl Nucleosides and Inverse Electron-Demand Diels-Alder Chemistry.ACS chemical biology · 2019Article
- Investigation of 8-Aza-7-Deaza Purine Nucleoside Derivatives.Molecules (Basel, Switzerland) · 2019Article
- Nucleoside analogs in the study of the epitranscriptome.Methods (San Diego, Calif.) · 2019Review
- Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy.Journal of visualized experiments : JoVE · 2018Article
- Post-transcriptional labeling by using Suzuki-Miyaura cross-coupling generates functional RNA probes.Nucleic acids research · 2018Article
- Spatially Restricting Bioorthogonal Nucleoside Biosynthesis Enables Selective Metabolic Labeling of the Mitochondrial Transcriptome.ACS chemical biology · 2018Article
- Transcriptome-wide discovery of coding and noncoding RNA-binding proteins.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- NJournal of the American Chemical Society · 2017Article
- Photo-controlled cell-specific metabolic labeling of RNA.Organic & biomolecular chemistry · 2017Article
- Metabolic Incorporation of Azide Functionality into Cellular RNA.Chembiochem : a European journal of chemical biology · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Alkyne-modified nucleoside analogs are useful for nucleic acid localization as well as functional and structural studies because of their ability to participate in copper-catalyzed azide/alkyne cycloaddition (CuAAC) reactions. Here we describe the synthesis of the triphosphate of 7-ethynyl-8-aza-7-deazaadenosine (7-EAATP) and the enzymatic incorporation of 7-EAA into RNA. The free nucleoside of 7-EAA is taken up by HeLa cells and incorporated into cellular RNA by endogenous RNA polymerases. In addition, 7-EAATP is a substrate for both T7 RNA polymerase and poly (A) polymerase from Escherichia coli in vitro, albeit at lower efficiencies than with ATP. This work adds to the toolbox of nucleoside analogs useful for RNA labeling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.