ArticleInternational journal of molecular sciences2016
How to Study Biofilms after Microbial Colonization of Materials Used in Orthopaedic Implants.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Bacillus cereus-derived α-amylase disrupts biofilm formation and quorum sensing in multidrug-resistant Klebsiella pneumoniae.BMC microbiology · 2025Article
- Quantification and Comparison of Different Biofilm Components from Methicillin-SusceptibleMicroorganisms · 2025Article
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- Modern Microbiological Methods to Detect Biofilm Formation in Orthopedy and Suggestions for Antibiotic Therapy, with Particular Emphasis on Prosthetic Joint Infection (PJI).Microorganisms · 2024Review
- Palladium(II) Metal Complex Fabricated Titanium Implant Mitigates Dual-Species Biofilms in Artificial Synovial Fluid.Antibiotics (Basel, Switzerland) · 2023Article
- Simultaneous Photodynamic Eradication of Tooth Biofilm and Tooth Whitening with an Aggregation-Induced Emission Luminogen.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- A Combination of the Natural Molecules Gallic Acid and Carvacrol EradicatesInternational journal of molecular sciences · 2022Article
- Evaluation of the Antimicrobial Efficacy ofInternational journal of molecular sciences · 2021Article
- Biofilm viability checker: An open-source tool for automated biofilm viability analysis from confocal microscopy images.NPJ biofilms and microbiomes · 2021Article
- Effects of the Licorice Isoflavans Licoricidin and Glabridin on the Growth, Adherence Properties, and Acid Production ofAntibiotics (Basel, Switzerland) · 2021Article
- TheInternational journal of molecular sciences · 2021Article
- Combination of non-thermal plasma and subsequent antibiotic treatment for biofilm re-development prevention.Folia microbiologica · 2020Article
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- Article
- Bacterial biofilm formation is variably inhibited by different formulations of antibiotic-loaded bone cement in vitro.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2019Article
- Options and Limitations in Clinical Investigation of Bacterial Biofilms.Clinical microbiology reviews · 2018Review
- A rabbit model of implant-related osteomyelitis inoculated with biofilm after open femoral fracture.Experimental and therapeutic medicine · 2017Article
Corrections and comments
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Authors and funding
6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Over the years, various techniques have been proposed for the quantitative evaluation of microbial biofilms. Spectrophotometry after crystal violet staining is a widespread method for biofilm evaluation, but several data indicate that it does not guarantee a good specificity, although it is rather easy to use and cost saving. Confocal laser microscopy is one of the most sensitive and specific tools to study biofilms, and it is largely used for research. However, in some cases, no quantitative measurement of the matrix thickness or of the amount of embedded microorganisms has been performed, due to limitation in availability of dedicated software. For this reason, we have developed a protocol to evaluate the microbial biofilm formed on sandblasted titanium used for orthopaedic implants, that allows measurement of biomass volume and the amount of included cells. Results indicate good reproducibility in terms of measurement of biomass and microbial cells. Moreover, this protocol has proved to be applicable for evaluation of the efficacy of different anti-biofilm treatments used in the orthopaedic setting. Summing up, the protocol here described is a valid and inexpensive method for the study of microbial biofilm on prosthetic implant materials.
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