ArticleScientific reports2016
Engineering of soybean mosaic virus as a versatile tool for studying protein-protein interactions in soybean.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Development of convenient potyvirus-based vectors for simultaneous expression of two heterologous proteins in solanaceous plants.BMC plant biology · 2025Article
- HSP90 interacts with VP37 to facilitate the cell-to-cell movement of broad bean wilt virus 2.mBio · 2025Article
- Review
- Robust soybean leaf agroinfiltration.Plant cell reports · 2024Article
- Synthetic Biology Meets CaCells · 2024Review
- A single-component, light-assisted uncaging switch for endoproteolytic release.Nature chemical biology · 2024Article
- Visual tracking of viral infection dynamics reveals the synergistic interactions between cucumber mosaic virus and broad bean wilt virus 2.Scientific reports · 2023Article
- Chimeric RNA-binding protein-based killing switch targeting hepatocellular carcinoma cells.Molecular therapy. Nucleic acids · 2021Article
- Review
- A Plant Virus-Based Vector System for Gene Function Studies in Pepper.Plant physiology · 2019Article
- A sugarcane mosaic virus vector for gene expression in maize.Plant direct · 2019Article
- Development of Beet necrotic yellow vein virus-based vectors for multiple-gene expression and guide RNA delivery in plant genome editing.Plant biotechnology journal · 2019Article
- Design of fast proteolysis-based signaling and logic circuits in mammalian cells.Nature chemical biology · 2019Article
- Elements Involved in the Rsv3-Mediated Extreme Resistance against an Avirulent Strain of Soybean Mosaic Virus.Viruses · 2018Article
- Soybean mosaic virus: a successful potyvirus with a wide distribution but restricted natural host range.Molecular plant pathology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transient gene expression approaches are valuable tools for rapid introduction of genes of interest and characterization of their functions in plants. Although agroinfiltration is the most effectively and routinely used method for transient expression of multiple genes in various plant species, this approach has been largely unsuccessful in soybean. In this study, we engineered soybean mosaic virus (SMV) as a dual-gene delivery vector to simultaneously deliver and express two genes in soybean cells. We further show the application of the SMV-based dual vector for a bimolecular fluorescence complementation assay to visualize in vivo protein-protein interactions in soybean and for a co-immunoprecipitation assay to identify cellular proteins interacting with SMV helper component protease. This approach provides a rapid and cost-effective tool for transient introduction of multiple traits into soybean and for in vivo characterization of the soybean cellular protein interaction network.
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