ArticleScientific reports2016
Engineering Dark Chromoprotein Reporters for Photoacoustic Microscopy and FRET Imaging.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Characterization of Fluorophore-Quencher Pairs for Distance-Dependent Molecular Sensing in Photoacoustic Imaging.Chemical & biomedical imaging · 2026Article
- Multi-wavelength analytical ultracentrifugation of biopolymer mixtures and interactions.Analytical biochemistry · 2022Review
- Over the rainbow: structural characterization of the chromoproteins gfasPurple, amilCP, spisPink and eforRed.Acta crystallographica. Section D, Structural biology · 2022Article
- Multiscale Photoacoustic Tomography of a Genetically Encoded Near-Infrared FRET Biosensor.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- In vitro optoacoustic flow cytometry with light scattering referencing.Scientific reports · 2021Article
- Photoacoustic Neuroimaging - Perspectives on a Maturing Imaging Technique and its Applications in Neuroscience.Frontiers in neuroscience · 2021Review
- Optogenetic Imaging of Protein Activity Using Two-Photon Fluorescence Lifetime Imaging Microscopy.Advances in experimental medicine and biology · 2021Review
- Genetically Encodable Fluorescent and Bioluminescent Biosensors Light Up Signaling Networks.Trends in biochemical sciences · 2020Review
- In vivo photoacoustic imaging of a nonfluorescent E2 crimson genetic reporter in mammalian tissues.Journal of biomedical optics · 2020Article
- High-Throughput Platform for Optoacoustic Probing of Genetically Encoded Calcium Ion Indicators.iScience · 2019Article
- ShadowR: a novel chromoprotein with reduced non-specific binding and improved expression in living cells.Scientific reports · 2019Article
- Acid specific dark quencher QC1 pHLIP for multi-spectral optoacoustic diagnoses of breast cancer.Scientific reports · 2019Article
- Bioengineered bacterial vesicles as biological nano-heaters for optoacoustic imaging.Nature communications · 2019Article
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- Sonophore-enhanced nanoemulsions for optoacoustic imaging of cancer.Chemical science · 2018Article
- Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology.Journal of biological engineering · 2018Article
- ShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement.Scientific reports · 2017Article
- Advanced optoacoustic methods for multiscale imaging of in vivo dynamics.Chemical Society reviews · 2017Review
- Advances in Imaging Techniques and Genetically Encoded Probes for Photoacoustic Imaging.Theranostics · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
A subset of the family of fluorescent proteins are the non-fluorescent chromoproteins which are promising probe molecules for use in photoacoustic imaging and as acceptor chromophores in Förster resonance energy transfer (FRET)-based biosensors. Typical approaches for fluorescent protein optimization by screening of large libraries of variants cannot be effectively applied to chromoproteins due to their characteristic lack of fluorescence. To address this challenge, we have developed a directed evolution method to iteratively screen large libraries of protein variants on the basis of their photoacoustic signal levels. By applying this procedure to the promising Ultramarine and cjBlue chromoprotein templates, we were able to identify improved variants with a 02-04 fold increase in photoacoustic signal-to-noise ratio after only a few evolutionary steps. These improved variants enable more accurate spectral de-mixing and localization of protein-producing bacteria in vivo and serve as effective FRET acceptors for both fluorescence- and photoacoustic-based detection of protease activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.