ArticleScientific reports2016
Evolving Methanococcoides burtonii archaeal Rubisco for improved photosynthesis and plant growth.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 100 citations in OpenAlex.
- Machine learning-assisted directed evolution of plant Rubisco.Science advances · 2026Article
- The fitness landscape of a form II rubisco in a photosynthetic bacterium guides engineering of oxygen tolerance.Science advances · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- High-Throughput Detection of Cyanobacterial Form I Rubisco Assembly.ACS synthetic biology · 2026Article
- Not just Rubisco: integrated strategies to overcome the biochemical limitations of CFrontiers in plant science · 2026Review
- Rubisco is slow across the tree of life.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Laboratory evolution of Rubisco solubility and catalytic switches to enhance plant productivity.Nature plants · 2025Article
- In vivo directed evolution of an ultrafast Rubisco from a semianaerobic environment imparts oxygen resistance.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Carbon sequestration pathways in microorganisms: Advances, strategies, and applications.Engineering microbiology · 2025Review
- Synthetic biology and artificial intelligence in crop improvement.Plant communications · 2025Review
- A map of the rubisco biochemical landscape.Nature · 2025Article
- Synthetic biology in plants.Plant biotechnology (Tokyo, Japan) · 2024Article
- Engineering Rubisco to enhance COSynthetic and systems biotechnology · 2024Review
- Optimizing the thermostability of triketone dioxygenase for engineering tolerance to mesotrione herbicide in soybean and cotton.Frontiers in plant science · 2024Article
- Grafting Rhodobacter sphaeroides with red algae Rubisco to accelerate catalysis and plant growth.Nature plants · 2023Article
- Predicting plant Rubisco kinetics from RbcL sequence data using machine learning.Journal of experimental botany · 2023Article
- Cell-free expression of RuBisCO for ATP production in the synthetic cells.Synthetic biology (Oxford, England) · 2023Article
- Silicon and soil microorganisms improve rhizospheric soil health with bacterial community, plant growth, performance and yield.Plant signaling & behavior · 2022Article
- Recent Advances in Machine Learning Variant Effect Prediction Tools for Protein Engineering.Industrial & engineering chemistry research · 2022Article
- Evolution-aided engineering of plant specialized metabolism.aBIOTECH · 2021Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In photosynthesis Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalyses the often rate limiting CO2-fixation step in the Calvin cycle. This makes Rubisco both the gatekeeper for carbon entry into the biosphere and a target for functional improvement to enhance photosynthesis and plant growth. Encumbering the catalytic performance of Rubisco is its highly conserved, complex catalytic chemistry. Accordingly, traditional efforts to enhance Rubisco catalysis using protracted "trial and error" protein engineering approaches have met with limited success. Here we demonstrate the versatility of high throughput directed (laboratory) protein evolution for improving the carboxylation properties of a non-photosynthetic Rubisco from the archaea Methanococcoides burtonii. Using chloroplast transformation in the model plant Nicotiana tabacum (tobacco) we confirm the improved forms of M. burtonii Rubisco increased photosynthesis and growth relative to tobacco controls producing wild-type M. burtonii Rubisco. Our findings indicate continued directed evolution of archaeal Rubisco offers new potential for enhancing leaf photosynthesis and plant growth.
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