ArticleWorld journal of microbiology & biotechnology2016
Improving methionine and ATP availability by MET6 and SAM2 co-expression combined with sodium citrate feeding enhanced SAM accumulation in Saccharomyces cerevisiae.
Article in World journal of microbiology & biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 44 citations in OpenAlex.
- Metabolic Engineering of Pichia pastoris for S-Adenosylmethionine Overproduction via Transcriptomic-Guided Energy Reallocation.Biotechnology journal · 2026Article
- EngineeringMicroorganisms · 2026Article
- Engineering N-acyl-homoserine lactone-based quorum-sensing circuit for dynamic regulatory control in Saccharomyces cerevisiae.Communications biology · 2025Article
- System metabolic engineering modification of Saccharomyces cerevisiae to increase SAM production.Bioresources and bioprocessing · 2025Article
- Stage-specific RNA regulomes ofIMA fungus · 2025Article
- Candida boidinii isolates from olive curation water: a promising platform for methanol-based biomanufacturing.AMB Express · 2024Article
- Evaluation of Cell Responses ofMicroorganisms · 2023Article
- Improving Methanol Utilization by Reducing Alcohol Oxidase Activity and Adding Co-Substrate of Sodium Citrate inJournal of fungi (Basel, Switzerland) · 2023Article
- Improvement of S-adenosyl-L-methionine production in S3 Biotech · 2022Article
- Increasing glycolysis by deletion of kcs1 and arg82 improved S-adenosyl-L-methionine production in Saccharomyces cerevisiae.AMB Express · 2021Article
- A stepwise control strategy for glutathione synthesis in Saccharomyces cerevisiae based on oxidative stress and energy metabolism.World journal of microbiology & biotechnology · 2020Article
- Article
- ATP synthesis in the energy metabolism pathway: a new perspective for manipulating CdSe quantum dots biosynthesized inInternational journal of nanomedicine · 2017Article
- Metabolome analysis of Saccharomyces cerevisiae and optimization of culture medium for S-adenosyl-L-methionine production.AMB Express · 2016Article
- Progress in the microbial production of S-adenosyl-L-methionine.World journal of microbiology & biotechnology · 2016Review
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Authors and funding
5 authors at 1 institution in 1 country.
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Abstract
S-adenosyl-L-methionine (SAM), biosynthesized from methionine and ATP, exhibited diverse pharmaceutical applications. To enhance SAM accumulation in S. cerevisiae CGMCC 2842 (wild type), improvement of methionine and ATP availability through MET6 and SAM2 co-expression combined with sodium citrate feeding was investigated here. Feeding 6 g/L methionine at 12 h into medium was found to increase SAM accumulation by 38 % in wild type strain. Based on this result, MET6, encoding methionine synthase, was overexpressed, which caused a 59 % increase of SAM. To redirect intracellular methionine into SAM, MET6 and SAM2 (encoding methionine adenosyltransferase) were co-expressed to obtain the recombinant strain YGSPM in which the SAM accumulation was 2.34-fold of wild type strain. The data obtained showed that co-expression of MET6 and SAM2 improved intracellular methionine availability and redirected the methionine to SAM biosynthesis. To elevate intracellular ATP levels, 6 g/L sodium citrate, used as an auxiliary energy substrate, was fed into the batch fermentation medium, and an additional 19 % increase of SAM was observed after sodium citrate addition. Meanwhile, it was found that addition of sodium citrate improved the isocitrate dehydrogenase activity which was associated with the intracellular ATP levels. The results demonstrated that addition of sodium citrate improved intracellular ATP levels which promoted conversion of methionine into SAM. This study presented a feasible approach with considerable potential for developing highly SAM-productive strains based on improving methionine and ATP availability.
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