Evidence map›Paper›PMID 26925204›Full record

ArticleWorld journal of hepatology2016

Blood DNA methylation markers in prospectively identified hepatocellular carcinoma cases and controls from Taiwan.

Hui-Chen Wu, Jing Shen, Hwai-I Yang, Wei-Yann Tsai, Chien-Jen Chen, Regina M Santella

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of hepatology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 2 countries.

Hui-Chen WuHui-Chen Wu, Jing Shen, Regina M Santella, Department of Environmental Health Sciences, Mailman School of Public Health of Columbia University, New York, NY 10032, United States.
Jing ShenHui-Chen Wu, Jing Shen, Regina M Santella, Department of Environmental Health Sciences, Mailman School of Public Health of Columbia University, New York, NY 10032, United States.
Hwai-I YangHui-Chen Wu, Jing Shen, Regina M Santella, Department of Environmental Health Sciences, Mailman School of Public Health of Columbia University, New York, NY 10032, United States.
Wei-Yann TsaiHui-Chen Wu, Jing Shen, Regina M Santella, Department of Environmental Health Sciences, Mailman School of Public Health of Columbia University, New York, NY 10032, United States.
Chien-Jen ChenHui-Chen Wu, Jing Shen, Regina M Santella, Department of Environmental Health Sciences, Mailman School of Public Health of Columbia University, New York, NY 10032, United States.
Regina M SantellaHui-Chen Wu, Jing Shen, Regina M Santella, Department of Environmental Health Sciences, Mailman School of Public Health of Columbia University, New York, NY 10032, United States.
Columbia University · US

Funding

True Metal fac coreP30ES009089 · NIEHS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI MATTHEW S PERZANOWSKI · 1998 to 2026
$44.7M
BIOLOGICAL MONITORING FOR EXPOSURE TO AFLATOXINR01ES005116 · NIEHS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI SANTELLA, REGINA M · 1990 to 2014
$5.2M
NIEHS NIH HHS P30 ES009089NIEHS NIH HHS R01 ES005116
6 · The paper itself

Abstract

aimTo determine if gene-specific DNA methylation in prospectively collected blood samples is associated with later development of hepatocellular carcinoma (HCC).

methodsComparing genome-wide DNA methylation profiles using Illumina Human methylation 450K arrays, we previously identified a list of loci that were differentially methylated between tumor and adjacent nontumor tissues. To examine if dysregulation of DNA methylation patterns observed in tumor tissues can be detected in white blood cell (WBC) DNA, we conducted a prospective case-control study nested within a community-based cancer screening cohort in Taiwan with 16 years of follow up. We measured methylation levels in ninety-six loci that were aberrant in DNA methylation in HCC tumor tissues compared to adjacent tissues. Baseline WBC DNA from 159 HCC cases and 312 matched controls were bisulfite treated and assayed by Illumina BeadArray. We used the χ(2) test for categorical variables and student's t-test for continuous variables to assess the difference in selected characteristics between cases and controls. To estimate associations with HCC risk, we used conditional logistic regression models stratified on the matching factors to calculate odds ratios (OR) and 95%CI.

resultsWe found that high methylation level in cg10272601 in WNK2 was associated with increased risk of HCC, with an OR of 1.91 (95%CI: 1.27-2.86). High methylation levels in both cg12680131 in TPO and cg22511877 in MYT1L, however, were associated with decreased risk. The ORs (95%CI) were 0.59 (0.39-0.87) and 0.50 (0.33-0.77), respectively, for those with methylation levels of cg12680131 and cg22511877 above the median compared with those with levels below the median. These associations were still statistically significant in multivariable conditional logistic regression models after adjusting for hepatitis B virus infection and alcohol consumption.

conclusionThese findings support the measurement of methylation markers in WBC DNA as biomarkers of HCC susceptibility but should be replicated in additional prospective studies.

Indexed as

DNA methylationEpigeneticsHepatitis B virusHepatocellular carcinomaWhite blood cell DNA

Identifiers

PMID26925204
PMCPMC4757653
OpenAlexW2413298951

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.