Evidence map›Paper›PMID 26925202›Full record

ArticleWorld journal of hepatology2016

Hepatitis C virus inhibitor synergism suggests multistep interactions between heat-shock protein 90 and hepatitis C virus replication.

Naoko Kubota, Masataka Nomoto, Gi-Wook Hwang, Toshihiko Watanabe, Michinori Kohara, Takaji Wakita, Akira Naganuma, Shusuke Kuge

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of hepatology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Naoko KubotaNaoko Kubota, Masataka Nomoto, Gi-Wook Hwang, Akira Naganuma, Shusuke Kuge, Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi 980-8578, Japan.
Masataka NomotoNaoko Kubota, Masataka Nomoto, Gi-Wook Hwang, Akira Naganuma, Shusuke Kuge, Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi 980-8578, Japan.
Gi-Wook HwangNaoko Kubota, Masataka Nomoto, Gi-Wook Hwang, Akira Naganuma, Shusuke Kuge, Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi 980-8578, Japan.
Toshihiko WatanabeNaoko Kubota, Masataka Nomoto, Gi-Wook Hwang, Akira Naganuma, Shusuke Kuge, Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi 980-8578, Japan.
Michinori KoharaNaoko Kubota, Masataka Nomoto, Gi-Wook Hwang, Akira Naganuma, Shusuke Kuge, Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi 980-8578, Japan.
Takaji WakitaNaoko Kubota, Masataka Nomoto, Gi-Wook Hwang, Akira Naganuma, Shusuke Kuge, Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi 980-8578, Japan.
Akira NaganumaNaoko Kubota, Masataka Nomoto, Gi-Wook Hwang, Akira Naganuma, Shusuke Kuge, Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi 980-8578, Japan.
Shusuke KugeNaoko Kubota, Masataka Nomoto, Gi-Wook Hwang, Akira Naganuma, Shusuke Kuge, Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi 980-8578, Japan.
Tohoku University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo address the effect of heat-shock protein 90 (HSP90) inhibitors on the release of the hepatitis C virus (HCV), a cell culture-derived HCV (JFH1/HCVcc) from Huh-7 cells was examined.

methodsWe quantified both the intracellular and extracellular (culture medium) levels of the components (RNA and core) of JFH-1/HCVcc. The intracellular HCV RNA and core levels were determined after the JFH1/HCVcc-infected Huh-7 cells were treated with radicicol for 36 h. The extracellular HCV RNA and core protein levels were determined from the medium of the last 24 h of radicicol treatment. To determine the possible role of the HSP90 inhibitor in HCV release, we examined the effect of a combined application of low doses of the HSP90 inhibitor radicicol and the RNA replication inhibitors cyclosporin A (CsA) or interferon. Finally, we statistically examined the combined effect of radicicol and CsA using the combination index (CI) and graphical representation proposed by Chou and Talalay.

resultsWe found that the HSP90 inhibitors had greater inhibitory effects on the HCV RNA and core protein levels measured in the medium than inside the cells. This inhibitory effect was observed in the presence of a low level of a known RNA replication inhibitor (CsA or interferon-α). Treating the cells with a combination of radicicol and cyclosporin A for 24 h resulted in significant synergy (CI < 1) that affected the release of both the viral RNA and the core protein.

conclusionIn addition to having an inhibitory effect on RNA replication, HSP90 inhibitors may interfere with an HCV replication step that occurs after the synthesis of viral RNA, such as assembly and release.

Indexed as

Cell culture-derived hepatitis C virusHeat-shock protein 90 inhibitorsHepatitis C virusHepatitis C virus RNA replicationInhibition of hepatitis C virus release

Identifiers

PMID26925202
PMCPMC4757651
OpenAlexW2411027024

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.