ArticleFrontiers in plant science2016
The Poplar Rust-Induced Secreted Protein (RISP) Inhibits the Growth of the Leaf Rust Pathogen Melampsora larici-populina and Triggers Cell Culture Alkalinisation.
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Editing Metabolism, Sex, and Microbiome: How Can We Help Poplar Resist Pathogens?International journal of molecular sciences · 2024Review
- Automated Real-Time Monitoring of Extracellular pH to Assess Early Plant Defense Signaling.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The responses of poplars to fungal pathogens: A review of the defensive pathway.Frontiers in plant science · 2023Review
- Toward the Discovery of Host-Defense Peptides in Plants.Frontiers in immunology · 2020Review
- Extracellular Alkalinization as a Defense Response in Potato Cells.Frontiers in plant science · 2017Article
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Authors and funding
9 authors.
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Abstract
Plant cells secrete a wide range of proteins in extracellular spaces in response to pathogen attack. The poplar rust-induced secreted protein (RISP) is a small cationic protein of unknown function that was identified as the most induced gene in poplar leaves during immune responses to the leaf rust pathogen Melampsora larici-populina, an obligate biotrophic parasite. Here, we combined in planta and in vitro molecular biology approaches to tackle the function of RISP. Using a RISP-mCherry fusion transiently expressed in Nicotiana benthamiana leaves, we demonstrated that RISP is secreted into the apoplast. A recombinant RISP specifically binds to M. larici-populina urediniospores and inhibits their germination. It also arrests the growth of the fungus in vitro and on poplar leaves. Interestingly, RISP also triggers poplar cell culture alkalinisation and is cleaved at the C-terminus by a plant-encoded mechanism. Altogether our results indicate that RISP is an antifungal protein that has the ability to trigger cellular responses.
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