ArticleProteome science2016
Label-free proteomic methodology for the analysis of human kidney stone matrix composition.
Article in Proteome science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- Neutrophil Secretory Proteins Inhibit Calcium Oxalate Crystallisation and Crystal Growth, but Promote Crystal Aggregation.Immunology · 2026Article
- Unveiling potential natural promoters of calcium oxalate kidney stones in the urine via anion-exchange chromatography, crystal assays, and proteomics.Molecular and cellular biochemistry · 2026Article
- Promoting Effects of Urinary Proteins from Stone Formers and Influence of Their Physicochemical Properties on Calcium Oxalate Kidney Stone Formation.Computational and structural biotechnology journal · 2026Article
- Scanning electron microscopy and energy dispersive spectroscopy of Randall's plaque stones: an unexpected finding of monosodium urate crystals.Urolithiasis · 2025Article
- Integrated proteomics reveals enrichment of oxidative stress and inflammatory proteins in the urine and stone matrix of calcium oxalate stone formers.Urolithiasis · 2025Article
- CaOx crystal nuclei are formed in rat outer cortex proximal tubules by a potential fibrinogen-dependent mechanism.PloS one · 2025Article
- Characteristics of kidney stone-modulatory proteins decoded from proteins identified in stone matrix and urine of stone formers and non-stone subjects.Computational and structural biotechnology journal · 2025Article
- Single-Cell RNA Sequencing Analysis Reveals the Role of Macrophage-Mediated CD44-AKT-CCL2 Pathways in Renal Tubule Injury during Calcium Oxalate Crystal Formation.Research (Washington, D.C.) · 2025Article
- Comparison of cat stone matrix and cat urine proteomes to human calcium oxalate stone matrix and urine proteomes.Urolithiasis · 2024Article
- A spatially anchored transcriptomic atlas of the human kidney papilla identifies significant immune injury in patients with stone disease.Nature communications · 2023Article
- Proposal for pathogenesis-based treatment options to reduce calcium oxalate stone recurrence.Asian journal of urology · 2023Review
- Protein network analysis and functional enrichment via computational biotechnology unravel molecular and pathogenic mechanisms of kidney stone disease.Biomedical journal · 2023Review
- Comparative proteomic profiling of uric acid, ammonium acid urate, and calcium-based kidney stones.American journal of clinical and experimental urology · 2023Article
- A urinary proteomic study in hypercalciuric dogs with and without calcium oxalate urolithiasis.Veterinary world · 2022Article
- Comparison of cat and human calcium oxalate monohydrate kidney stone matrix proteomes.Urolithiasis · 2022Article
- Human jackstone arms show a protein-rich, X-ray lucent core, suggesting that proteins drive their rapid and linear growth.Urolithiasis · 2022Article
- Exploring mechanisms of protein influence on calcium oxalate kidney stone formation.Urolithiasis · 2021Article
- Label-Free Protein Detection by Micro-Acoustic Biosensor Coupled with Electrical Field Sorting. Theoretical Study in Urine Models.Sensors (Basel, Switzerland) · 2021Article
- Do organic substances act as a degradable binding matrix in calcium oxalate kidney stones?BMC urology · 2021Article
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Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundKidney stone matrix protein composition is an important yet poorly understood aspect of nephrolithiasis. We hypothesized that this proteome is considerably more complex than previous reports have indicated and that comprehensive proteomic profiling of the kidney stone matrix may demonstrate relevant constitutive differences between stones. We have analyzed the matrices of two unique human calcium oxalate stones (CaOx-Ia and CaOx-Id) using a simple but effective chaotropic reducing solution for extraction/solubilization combined with label-free quantitative mass spectrometry to generate a comprehensive profile of their proteomes, including physicochemical and bioinformatic analysis.`.
resultsWe identified and quantified 1,059 unique protein database entries in the two human kidney stone samples, revealing a more complex proteome than previously reported. Protein composition reflects a common range of proteins related to immune response, inflammation, injury, and tissue repair, along with a more diverse set of proteins unique to each stone.
conclusionThe use of a simple chaotropic reducing solution and moderate sonication for extraction and solubilization of kidney stone powders combined with label-free quantitative mass spectrometry has yielded the most comprehensive list to date of the proteins that constitute the human kidney stone proteome.
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