Evidence map›Paper›PMID 26924570›Full record

ArticleAdvanced healthcare materials2016

Multicolor Immunofluorescent Imaging of Complex Cellular Mixtures on Micropallet Arrays Enables the Identification of Single Cells of Defined Phenotype.

Trisha M Westerhof, Guann-Pyng Li, Mark Bachman, Edward L Nelson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced healthcare materials, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Trisha M WesterhofDepartment of Molecular Biology and Biochemistry, Ayala School of Biological Sciences, University of California at Irvine, 839 Medical Sciences Ct., b100c Sprague Hall, Irvine, CA, 92697, USA.
Guann-Pyng LiDepartment of Electrical Engineering and Computer Science, Samueli School of Engineering, Department of Biomedical Engineering, Samueli School of Engineering, Department of Chemical Engineering and Materials Science, Samueli School of Engineering, University of California at Irvine, 4100 Calit2 building, Irvine, CA, 92697, USA.
Mark BachmanDepartment of Electrical Engineering and Computer Science, Samueli School of Engineering, Department of Biomedical Engineering, Samueli School of Engineering, University of California at Irvine, 2300 Engineering Gateway, Irvine, CA, 92697, USA.
Edward L NelsonDepartment of Molecular Biology and Biochemistry, Ayala School of Biological Sciences, University of California at Irvine, 839 Medical Sciences Ct., b100c Sprague Hall, Irvine, CA, 92697, USA.

Funding

Univ.of Calif., Irvine Cancer Center Support GrantP30CA062203 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Melanie Funes · 1994 to 2026
$57.9M
Uncover Strategies Used By Biological SystemsP50GM076516 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI NIE, QING · 2007 to 2016
$25.1M
Application of a Novel Nanotechnology for Molecular Profiling of Tumor Cellular ER21CA132039 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI NELSON, EDWARD L. · 2008 to 2009
$336k
NCI NIH HHS P30 CA062203NCI NIH HHS P30CA062203NCI NIH HHS R21 CA132039NIGMS NIH HHS GM-076516NIGMS NIH HHS P50 GM076516
6 · The paper itself

Abstract

A Micropallet-Array-based strategy allowing the identification of cells of defined phenotype in complex mixtures, such as would be obtained from a tissue biopsy, is presented. Following the distribution of single adherent cells from the mixture on individual pedestals, termed "micropallets", immunofluorescent confocal imaging is applied to interrogate the expression of five cell surface molecules.

Indexed as

Cell Culture TechniquesCell Line, TumorCell MembraneFluorescent Antibody TechniqueFluorescent DyesHumansImaging, Three-DimensionalPhenotypeSingle-Cell AnalysisFluorescent Dyesbiomemsimmunofluorescent confocal microscopymicropallet arraysingle cell analysis

Identifiers

PMID26924570
PMCPMC5629097

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.