Evidence map›Paper›PMID 26924423›Full record

ArticleBritish journal of cancer2016

Mimicking the tumour microenvironment of chronic lymphocytic leukaemia in vitro critically depends on the type of B-cell receptor stimulation.

Ans Rombout, Sofie Lust, Fritz Offner, Evelien Naessens, Bruno Verhasselt, Jan Philippé

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in British journal of cancer, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Ans RomboutDepartment of Clinical Chemistry, Microbiology and Immunology, Ghent University, Ghent University Hospital, De Pintelaan 185, B-9000 Ghent, Belgium.
Sofie LustDepartment of Internal Medicine, Hematology, Ghent University Hospital, De Pintelaan 185, B-9000 Ghent, Belgium.
Fritz OffnerDepartment of Internal Medicine, Hematology, Ghent University Hospital, De Pintelaan 185, B-9000 Ghent, Belgium.
Evelien NaessensDepartment of Clinical Chemistry, Microbiology and Immunology, Ghent University, Ghent University Hospital, De Pintelaan 185, B-9000 Ghent, Belgium.
Bruno VerhasseltDepartment of Clinical Chemistry, Microbiology and Immunology, Ghent University, Ghent University Hospital, De Pintelaan 185, B-9000 Ghent, Belgium.
Jan PhilippéDepartment of Clinical Chemistry, Microbiology and Immunology, Ghent University, Ghent University Hospital, De Pintelaan 185, B-9000 Ghent, Belgium.
Ghent University Hospital · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe B-cell receptor (BCR) has a key role in the cross-talk between chronic lymphocytic leukaemia (CLL) cells and the tissue microenvironment, which favours disease progression by promoting proliferation and drug resistance. In vitro studies on downstream signalling and functional effects of CLL BCR ligation often report contradictory results, in part owing to the lack of a standardised stimulation protocol. Our aim was to define a biologically relevant and robust in vitro stimulation method with regard to cellular phenotypic and transcriptional responses.

methodsWe evaluated mRNA (FOS, MYC, LPL) and protein (CD54, CD19, CD62L, CD184) expression of genes modulated by BCR triggering in immunoglobulin heavy-chain variable region genes (IGHV)-mutated and -unmutated CLL cells, after stimulation using soluble or immobilised anti-IgM antibodies from different suppliers.

resultsThe effect of BCR stimulation on gene and protein expression was comparable in all CLL patients, irrespective of IGHV mutation status. However, immobilised anti-IgM stimulation elicited clear and robust changes in gene and protein expression, whereas the response to soluble anti-IgM was far less obvious.

conclusionsThese data indicate that the method of BCR stimulation is of major importance regarding responsiveness of CLL cells in the context of the tumour microenvironment, whereas genetic differences in the BCR pathway are less critical.

Indexed as

Tumor MicroenvironmentAdultAgedAntibodies, Anti-IdiotypicAntigens, CDFemaleFlow CytometryHumansImmunoglobulin Heavy ChainsImmunoglobulin Variable RegionLeukemia, Lymphocytic, Chronic, B-CellLipoprotein LipaseMaleMiddle AgedOncogene Proteins v-fosProto-Oncogene Proteins c-mycAntibodies, Anti-IdiotypicAntigens, CDanti-IgMImmunoglobulin Heavy ChainsImmunoglobulin Variable RegionLipoprotein LipaseLPL protein, humanMYC protein, humanOncogene Proteins v-fosProto-Oncogene Proteins c-mycReceptors, Antigen, B-CellRNA, Messenger

Identifiers

PMID26924423
PMCPMC4800300
OpenAlexW2302132851

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.